鸭心苹果酸脱氢酶功能基团的研究  

Functional Groups of Malate Dehydrogenase from Duck Heart

作　　者：孙芳[1] 王红扬[1] 王洁[1] 李星[1] 唐云明[1] 

机构地区：[1]西南大学生命科学学院 重庆市甘薯工程研究中心 三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《食品科学》2014年第15期212-215,共4页Food Science

基　　金：重庆市科委重点攻关项目(2011AB1027)

摘　　要：分别用对氯汞苯甲酸、苯甲基磺酰氟、N-溴代琥珀酰亚胺、琥珀酸酐、乙酰丙酮、溴代乙酸、N-乙酰咪唑、二巯基苏糖醇、氯胺-T等化学修饰剂对鸭心苹果酸脱氢酶进行修饰。结果表明:半胱氨酸巯基、色氨酸侧链吲哚基和丝氨酸羟基是鸭心细胞质苹果酸脱氢酶和线粒体苹果酸脱氢酶发挥活性的必需基团;而甲硫氨酸的硫醚基、组氨酸的咪唑基、精氨酸胍基、二硫键、酪氨酸的酚羟基、赖氨酸的ε-氨基与鸭心苹果酸脱氢酶活性不直接相关,不是酶活性中心的必需基团。The chemical modification of malate dehydrogenase(MDH) with p-chloromercuribenzoate, phenylmethyl sulfonyl fluoride, N-bromosuccinimide, succinic anhydride, acetylacetone, bromoacetic acid, N-acetylimidazole, dithiothreitol and chloramine-T showed that the cysteine sulfydryl, tryptophan side chain indole and serine hydroxyl groups involved in the composition of the enzyme active center could be considered as the essential functional groups of c-MDH and m-MDH from duck heart. However, the thioether group of methionine, the imidazole group of histidine, the guanidine group of arginine, disulfide bond, the phenol hydroxyl group of tyrosine and the epsilon-amino group of lysine were not directly related to the activity of the two MDH enzymes from duck heart, suggesting that they are not essential for the enzyme active center.

关 键 词：鸭心 苹果酸脱氢酶 功能基团 化学修饰 

分 类 号：S834[农业科学—畜牧学]