QuEChERS-高效液相色谱-质谱法检测食品中14种真菌毒素  

Simultaneous Screening for 14 Mycotoxin Contaminants in Foods by QuEChERS-LC-MS-MS

作　　者：史娜[1,2] 侯彩云[1] 路勇[2] 姜杰[2] 张学亮[3] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京市食品安全监控中心,北京100041 [3]北京工业大学生命科学与生物工程学院,北京100124

出　　处：《食品科学》2014年第16期190-196,共7页Food Science

摘　　要：建立QuEChERS-高效液相色谱-质谱检测食品中14种真菌毒素的方法。均质样品用1%乙酸-乙腈提取,经分散固相萃取净化后,采用ACQUITU UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7 μm)分离。采用电喷雾电离、多反应监测方式,可同时对食品中脱氧雪腐镰刀菌烯醇、青霉酸、黄曲霉毒素M1、黄曲霉毒素G2、桔青霉毒素、黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、玉米赤霉烯酮、赭曲霉毒素A、杂色曲霉毒素、HT-2毒素、T-2毒素、鬼臼毒素14种真菌毒素进行定性和定量分析。最低检出限为0.5~1 μg/kg。该方法简便快速、选择性佳、灵敏度高,适用于食品安全事件分析中真菌毒素的分析。This paper presents a QuEChERS-liquid chromatography-tandem mass chromatography (LC-MS-MS) method for the simultaneous screening for 14 mycotoxin contaminants (deoxynivalenol, penicillic acid, aflatoxin M1, aflatoxin G2, citrinin, aflatoxin B1, aflatoxin B2, aflatoxin G1, zearalenone, ochratoxin A, sterigmatocystin, HT-2 toxin, T-2 toxin and podophyllotoxin) in foods. The homogenized samples were extracted with 1% acetic acid in acetonitrile. The separation was performed on an ACQUITU UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) by gradient elution after purification by dispersive-solid-phase extraction (d-SPE). The 14 mycotoxin contaminants in foods analyzed by LC-MS-MS-ESI in the positive ionization, multiple-reaction monitoring (MRM) mode. The minimum detection limit was 0.5–1 μg/kg. The developed method proved to be simple, rapid, highly selective and sensitive, and suitable for the analysis of mycotoxin contaminants in food safety incidents.

关 键 词：真菌毒素 QUECHERS 高效液相色谱-质谱 筛选 食品 

分 类 号：O657.63[理学—分析化学] TS207.4[理学—化学]