响应面法优化小刺青霉16-7产纤维素酶液体发酵工艺  

Optimization of Cellulase Production by Penicillium spinulosum 16-7 in Liquid-State Fermentation by Response Surface Methodology

作　　者：李悦[1] 薛桥丽[2] 李世俊[1] 王晶 胡永金[1] 

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南农业大学图书馆,云南昆明650201 [3]玉溪沃森生物技术有限公司,云南玉溪653100

出　　处：《食品科学》2014年第17期137-145,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(30960308);云南省自然科学基金项目(2008ZC057M)

摘　　要：在单因素试验的基础上,采用Plackett-Burman试验设计及响应面分析,利用Minitab软件,对纤维素酶高产菌小刺青霉(Penicillium spinulosum)16-7进行发酵工艺条件的优化。通过Plackett-Burman试验筛选出影响产酶的3个主要因素,即稻草-麸皮(碳源)添加量、培养温度和培养时间。在此基础上通过最陡爬坡试验和响应面分析法进行回归分析。结果表明:当稻草-麸皮添加量为3.45 g/100 mL、培养温度为27.11℃和培养时间为146.27 h时,酶活力最高,此条件下滤纸酶酶活力预测值为132.53 U/mL。经过修正,选择稻草-麸皮添加量3.45 g/100 mL、培养温度27℃、培养时间146 h,此条件下测得羧甲基纤维素酶酶活力为387.58 U/mL、滤纸酶酶活力为128.86 U/mL,滤纸酶酶活力比优化前提高49.07%。This paper reports the optimization of the fermentation conditions for cellulase production by Penicillium spinulosum 16-7 by Plackett-Burman design and response surface methodology using the statistical software Minitab. By using Plackett-Burman design, carbon source(a combination of rice straw and wheat bran at a mass ratio of 7:3) concentration, culture temperature and time were identified as main factors that influence cellulase production. The selected variables were further investigated by steepest ascent analysis and optimized by response surface analysis. The highest cellulase activity was obtained after culture at 27.11 ℃ for 146.27 h at a carbon source concentration of 3.45 g/100 mL, leading to a predicted filter paper activity of 132.53 U/mL. Under the modified fermentation conditions of 3.45 g/100 mL carbon source concentration, 27 ℃ and 146 h, carboxymethyl cellulase activity was 387.58 U/mL and the filter paper activity was 128.86 U/mL, 49.07% higher than that obtained before optimization.

关 键 词：纤维素酶 发酵 Plackett-Bumann设计 响应面分析 小刺青霉 

分 类 号：TQ925[轻工技术与工程—发酵工程]