脱脂羊脑蛋白酶解条件优化及酶解产物体外抗氧化活性  

Optimization of Enzymatic Hydrolysis of Goat Brain Protein and Antioxidant Activity of the Resultant Hydrolysates

作　　者：常飞[1] 杨雪果[1] 肖士成 段旭昌[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]陕西杨凌华兴羊产业科技发展有限公司,陕西杨凌712100

出　　处：《食品科学》2015年第3期114-121,共8页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD28B05-3)

摘　　要：以脱脂羊脑蛋白为原料,采用响应面(response surface method,RSM)法建立脱脂羊脑蛋白的枯草芽孢杆菌中性蛋白酶水解回归模型,优化酶解工艺条件,在体外研究脱脂羊脑中性蛋白酶酶解产物的抗氧化性能。结果表明:脱脂羊脑蛋白底物质量浓度为3.03 g/100 mL,酶添加量为5 653.20 U/g,温度为39.4 ℃时,脱脂羊脑蛋白水解度最高,达到(14.59±1.26)%。当脱脂羊脑蛋白水解度为(14.39±1.17)%时,酶解产物对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟自由基(?OH)清除能力最强;当脱脂羊脑蛋白水解度为(12.48±0.71)%时 ,酶解产物对超氧阴离子自由基(O2-?)清除能力和总还原能力最强;当脱脂羊脑蛋白水解度为(12.48±0.71)%时,酶解产物对DPPH自由基、?OH、O2-?、亚硝酸根阴离子的IC50分别2.49、3.13、10.37、10.89 mg/mL,酶解产物对Fe2+螯合率的IC50为7.48 mg/mL,证明脱脂羊脑蛋白酶解产物具有一定抗氧化活性。The enzymatic hydrolysis parameters of defatted goat brain protein with Bacillus subtilis neutral protease were optimized by response surface methodology. The optimized parameters were determined as fol lows: substrate concentration,3.03 g/100 mL; enzyme/substrate ratio, 5 653. 20 U/g; and hydrolysis temperature, 39.4 ℃. The maximum degree of protein hydrolysis was (14.59±1.26) % with the optimized parameters. The antioxidant activity of the resultant hydrolysates was investigated in vitro. The antioxidant activity presented its maximum value when the hydrolysis degree was (12.48±0.71)%.The IC50 of the protein hydrolysates for scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide anion, and nitrite radicals were 2.49, 3.13, 10.37 and 10.89 mg/mL, respectively, and the IC50 for chelating Fe2+ ions was 7.48 mg/mL.These results suggest that the protein hydrolysates possess excellent antioxidant activity.

关 键 词：羊脑蛋白 酶解产物 响应面 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]