沙门氏菌噬菌体STP4-a重组内溶素抑菌特性分析  

Characterization of Recombinant Endolysin from a Salmonella-Infecting Bacteriophage STP4-a

作　　者：林洪[1] 李萌[1] 王静雪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2015年第5期104-108,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD28B05)

摘　　要：为研究内溶素重组蛋白Lys STP4对沙门氏菌的抑菌性能,采用扩散法和浊度法探讨不同p H值、温度、离子强度等条件对其裂解稳定性的影响。结果表明,Lys STP4在p H 5~10和温度30~50℃的条件下均能保持较高的活性,在-80℃条件下保存6个月仍有85%的残留酶活力,与膜通透剂乙二胺四乙酸(ethylenediaminetetraacetic acid,EDTA)协同作用能够有效抑制高浓度沙门氏菌的生长。In the present study,the recombinant endolysin from Salmonella-infecting bacteriophase STP4-a was characterized for its biochemical properties such as p H,temperature and iron concentration by the plate diffusion a n d turbidity methods.The results showed that the endolysin was stable over broad p H and temperature ranges and was active from p H 5.0 to 10 and from 30 to 50 ℃.After 6 months of storage at-80 ℃,the activity of endolysin retained 85% of the original level.Ethylenediaminetetraacetic acid(EDTA) was used to increase outer membrane permeability,and it greatly enhanced the lytic activity of STP4-a endolysin.

关 键 词：沙门氏菌 烈性噬菌体 内溶素 

分 类 号：TS201.3[轻工技术与工程—食品科学]