超声波辅助提取人参花多糖工艺优化及其抗氧化活性  

Ultrasonic-Assisted Extraction of Polysaccharides from Ginseng Flower and Their Antioxidant Activity in vitro

作　　者：周思思[1] 王榆元[2] 刘丹[1] 张一旻 刘思余 王晓晴[1] 曾晓雄[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南华大学化学化工学院,湖南衡阳421001

出　　处：《食品科学》2015年第6期76-81,共6页Food Science

基　　金：江苏省优势学科人才引进计划项目(08080900213);南京农业大学SRT计划项目(1318A10)

摘　　要：以干燥人参花为原料提取多糖,在单因素试验的基础上,利用响应面分析法优化超声波辅助提取人参花多糖工艺,并建立回归模型;同时探究其体外抗氧化活性。结果表明:超声波辅助提取人参花多糖的最佳提取工艺为超声功率586?W、超声时间18.65?min、料液比1∶19.75(g/m L),在此条件下多糖得率为(5.20±0.17)%(n=3)。超声提取的人参花多糖具有较高的抗氧化活性,对1,1-二苯基-2-三硝基苯肼自由基、羟自由基和超氧阴离子自由基清除作用明显,且其质量浓度与抗氧化活性呈现一定的量效关系,是一种良好的天然抗氧化剂。Based on single factor experiments,the ultrasonic-assisted extraction process for crude water-soluble polysaccharides(μGFL) from dry ginseng flower was optimized with response surface methodology.The in vitro antioxidant activity of polysaccharides extracted from ginseng flower was determined.The optimal extraction conditions were obtained as follows:ultrasonic power,586 W;extraction duration,18.65 min;and material/liquid ratio,1:19.75.Under these conditions,the extraction yield of polysaccharides from ginseng flower was(5.20 ± 0.17)%(n = 3).The ginseng flower polysaccharides showed potential scavenging activity against l,l-diphenyl-2-picrylhydrazxyl(DPPH),hydroxyl and superoxide anionradical in a concentration-dependent manner.This study indicates that ginseng flower polysaccharides are a good source of natural antioxidants.

关 键 词：人参花多糖 超声波 响应面 工艺优化 抗氧化 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]