反相高效液相色谱法测定乳制品中核苷酸方法的研究  

Research on the Determination Method of Nucleotides in Dairy by HPLC

作　　者：郝岩平[1] 姜金斗[1] 胡向蔚[2] 

机构地区：[1]国家乳制品质量监督检验中心,哈尔滨150086 [2]中国食品发酵工业研究院,北京100027

出　　处：《中国食品添加剂》2003年第2期114-118,共5页China Food Additives

摘　　要：建立了高效液相色谱法测定乳制品中核苷酸含量的方法。采用紫外检测器 ,波长为 2 5 4nm ;色谱柱为迪马钻石C18,柱温为 2 5℃ ,流动相为① 0 0 1mol/LK2 HPO496 0ml+0 1mol/LK2 HPO440mlpH5 ;测定腺嘌呤核苷 (AMP)、鸟嘌呤核苷 (GMP)、次黄嘌呤核苷 (IMP)。②乙腈 :(0 0 1mol/LKH2 PO4+0 0 0 5mol/LK2 HPO4+1mmol/L四丁基溴化氨 ,磷酸调 pH5 0 ) =5 :95 ,测定胞嘧啶核苷 (CMP)、脲嘧啶核苷 (CMP)。流速为 1 0ml/min。五种核苷酸组分。标准曲线相关系数r为CMP 0 986 8;UMP 0 9879;GMP 0 9792 ;IMP 0 9799;AMP0 95 5 3;回收率为 94 6 % ;8次样品重复测定变异系数为 1 79%。A method for determination of nucleotides in dairy by HPLC is reported Ultraviolete detector,Dikma-C 18 column was used with column temperature 25℃ Mobile phase ① 0 01mol/L KH 2PO 4960ml+0 1mol/L K 2HPO 440ml pH5 was used for AMP、GMP、IMP;②ACN:(0 01mol/L KH 2PO 4+0 005mol/L K 2HPO 4+1mmol/LC 16 H 36 BrN,pH5 0)=5:95 was used for CMP、UMP The speed of mobile phase was 1 0ml/min The relative coefficient was CMP 0 9868、UMP 0 9879、GMP 0 9792、IMP 0 9799、AMP 0 9553,the recovery was 94 6%;the CV in 8 sample testing was 1 79%

关 键 词：反相高效液相色谱法 测定方法 乳制品 核苷酸 婴幼儿配方奶粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]