培养条件对一株嗜酸乳杆菌突变株亚油酸异构酶活力的影响  

EFFECT OF CULTURE CONDITION ON LINOLEATE ISOMERASE ACTIVITIES OF A LACTOBACILLUS ACIDOPHILUS MUTANT

作　　者：曹健[1] 曾实[1] 魏明[1] 白爱英[1] 张浩[1] 

机构地区：[1]郑州工程学院生物工程系,河南郑州450052

出　　处：《郑州工程学院学报》2003年第4期14-17,共4页

基　　金：河南省杰出青年基金项目(04120001800);河南省科技厅科技攻关项目(001090126)

摘　　要：对一株嗜酸乳杆菌突变株产亚油酸异构酶的条件进行了研究,得到最佳产酶条件为:以脱脂乳作为培养基,添加3%的乳糖、1%的(NH4)2SO4、0 1%的亚油酸及1%的NaCl,接种量为1%,培养时间为36h.在此条件下,所合成的亚油酸异构酶活力达252 0U/mL.The condition of a lactobacillus acidophilus Mutant to synthesize linoleic isomerase was studied. The optimum conditions were as fellows: the proper medium was skim milk, with addition of 1%(V/V) lactose, 1%((W/V)) (NH_4)_2SO_4, 0.1%(V/V) linoleic acid and 1%(W/V) NaCl. The inoculum volume was 1%(V/V), and the cultivation period was 36 hrs. Linoleate isomerase activity reached 252.0U·mL under such condition.

关 键 词：嗜酸乳杆菌突变株 亚油酸异构酶 酶活力 产酶条件 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]