杜氏盐藻叶绿体转化载体pDS16S-CAT的构建  

Construction of transformation vector pDS16S-CAT for chloroplast of Dunaliella salina

作　　者：潘卫东[1] 袁保梅[1] 谢华[1] 牛向丽[1] 许培荣[1] 薛乐勋[1] 王建民[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第1期25-28,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省重大科技攻关基金资助项目 0 12 2 0 3 2 5 0 0 ;河南省杰出人才创新基金资助项目 0 2 2 10 0 190 0

摘　　要：目的 :利用同源重组方法 ,将外源基因氯霉素乙酰转移酶 (CAT)转入杜氏盐藻叶绿体中。方法 :以杜氏盐藻叶绿体 1 6SrRNA序列为同源片段 ,构建盐藻叶绿体表达载体 ,并利用基因枪法转化盐藻 ,使CAT基因得到表达。结果 :转化后的盐藻可以在含有氯霉素的培养基中生存 3～ 4周 ,表明CAT基因已转入盐藻叶绿体中并表达。结论 :盐藻叶绿体转化是可行的 ,为获得稳定转化的盐藻 。Aim: To transfer chloramphenicol acetyltransferase (CAT) gene to the chloroplast of Dunaliella salina by homologous recombination. Method: Cloned 16S rRNA sequence of the chloroplast of Dunaliella salina was used as homologous fragment to construct a transformation vector pDC16r-CAT which was transferred to the cells of Dunaliella salina, using microprojectile delivery. Results: The transformed strain of Dunaliella salina cells survived in media containing chloramphenicol for 3～4 weeks and the transformants exhibited obvious chloramphenicol resistance, suggesting that the CAT gene be transferred to the chloroplast of Dunaliella salina.Conclusion: It is available to transform the chloroplast of Dunaliella salina and correct homologous sequences are necessary for stable transformation.

关 键 词：盐藻 叶绿体 16S RRNA 转化载体 

分 类 号：Q244[生物学—细胞生物学] Q782