ELISA检测食品中黄曲霉毒素B_1的研究  

DETERMINATION OF AFLATOXIN B_1 BY ENZYME-LINKED IMMUNOSORBENT

作　　者：余礼碧[1] 张伯玲[1] 陈宁蓉 张坐奎[2] 罗发成 

机构地区：[1]成都军区军事医学研究所,610061 [2]四川大学原子核科学技术研究所,成都610064 [3]四川省卫生防疫站成都

出　　处：《西南农业学报》1992年第4期83-87,共5页Southwest China Journal of Agricultural Sciences

摘　　要：用自制人工抗原AFB1-Oxime-BSA免疫家兔制得了特异性强、灵敏度高的抗黄曲霉毒素B1（AFB1）血清,其效价达106以上。建立了间接双体酶联免疫吸附试验检测食品中AFB1,最小检测值5pg/0.1ml。对三类食品（50份样品）加标回收,其回收率为70～102％,变异系数为8～18％。将本法与TLC测得结果作统计学处理,P>0.05,说明两法测得结果的差异无显著性。本法简便、灵活、适于大批样品检测。An antiserum against aflatoxin B1 have been peen produced by immunizing rabbits with an artifl cial immunogen, aflatoxin B1-oxime-BSA conjugation. The antiserum has been used to set up an indirect, double antibody ELISA with a limit of detection of 5 Pg/0.1ml. The recoveries of aflatovin B to 3 kinds of samples spiked in the 10-100 PPb range is 70% to 102% with a coefficient of variation of 8-18% (n=50) . The values of aflatoxin B1 obtained in 24 corn samples using this method have been compared with the TLC. The difference between them is not significant in the view of statistics.

关 键 词：食品 黄曲霉毒素 ELISA 分析 

分 类 号：TS207.4[轻工技术与工程—食品科学]