黄桃水溶性多糖的提取和分离纯化  

Extraction Isolation and Purification of Water Soluble Polysaccharides from Peach

作　　者：陈留勇[1] 孟宪军[1] 孔秋莲[2] 宋立峰[3] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161 [2]上海农业科学院农产品加工保鲜中心,上海201106 [3]辽宁经济职业技术学院生物应用工程系,辽宁沈阳110122

出　　处：《食品科学》2004年第1期81-84,共4页Food Science

摘　　要：采用水提醇沉法从黄桃果肉中提取出水溶性多糖,经去蛋白和色素,得到纯白色的黄桃水溶性多糖HTP,过DEAE-纤维素柱分别在水洗脱和盐洗脱部分得到HTP1和HTP2,HTP1和HTP2过Sephadex G-300凝胶柱,仍为单一对称峰,采用凝胶色谱法和纸层析鉴定纯度,表明为纯品。The water soluble polysaccharides of peach were extracted in hot water, then isolated by ethanol. The proteinin polysaccharides was removed by Sevag way and the pigment in polysaccharides was removed by H2O2. The water-solublepolysaccharides of peach which color was pure white were got. HTP were further purified to HTP1 and HTP2 with DEAEcolumn chromatography. HTP1 and HTP2 were purified with Sephadex-300 column chromatography respectively, howeverwere singular peak. The gel filtration chromatography and paper chromatography proved that HTP1 and HTP2 were purematerial.

关 键 词：黄桃 水溶性多糖 提取 分离纯化 水提醇沉法 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]