酿造工业用果胶酶、纤维素酶生产菌的选育研究  

Study on Screening and Cultivation of Pectinase-, Cellulase- producing Strains in Brewing Industry

作　　者：谷海先[1] 王建[1] 

机构地区：[1]江南大学生物工程学院,江苏无锡214036

出　　处：《中国酿造》2004年第1期11-14,共4页China Brewing

摘　　要：该文以腐败香蕉等水果和各酒厂的大曲作为菌源,经筛选获得的黑曲霉563具有同时产果胶酶和纤维素酶的能力。再经紫外线诱变得到FV-4菌株,在麸皮:玉米芯=5:5、1%果胶溶液的培养基中于33℃条件下培养5d,果胶酶活力达到3750U/g(干基),纤维素酶活力达到1800U/g(干基)。The rotten fruits such as bananas and Daqu(koji) from wine plants were taken as source strains. The obtained Aspergillus niger 563 strain had the ability to produce pectinase and cellulase. The mutant FV-4 obtained through ultraviolet mutagenesis was cultivated at 33℃ for 5 d in the medium containing bran and cob at the ratio of 5:5, as well as 1% pectin solution. The result showed that the activity of peptinase and cellulase reached 3750 U/g (dry basis) and 1800 U/g (dry basis), respectively.

关 键 词：黑曲霉 诱变育种 固态发酵 果胶酶 纤维素酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]