酶法测定农药残留量的量热系统的初步实验研究  

Primary Experimental Research of Calorimetry on Pesticide Detection with Immobilized Enzyme

作　　者：赵肖磊[1] 郑艺华[1] 王芳芳[1] 华泽钊[1] 

机构地区：[1]上海理工大学制冷与低温技术研究所,上海200093

出　　处：《食品科学》2004年第2期127-130,共4页Food Science

基　　金：国家自然科学基金资助项目(50176032)

摘　　要：根据酶法检测原理,本文建立了用于农药残留检测的量热式流动注射分析系统,由蠕动泵、恒温器、酶反应室、热电堆、高精度万用表和电脑等组成。保持恒定温度的酶反应液在绝热反应室中进行反应。恒温是采用半导体制冷恒温器来实现的。通过实验,系统恒温效果较好;通过进行流动注射量热实验,可以检测到酶反应热,且重复性较好,说明该系统用于农药残留检测的可行性。A calorimetric flow injection analysis system for detection of pesticide residue is described based on the theory ofenzyme reaction. This system consists of a peristaltic pump, a thermostat, Thermocouple temperature sensor, a high precisionmultimeter and a computer. The reaction mix solution was controlled at constant temperature when it flows through thermostat,then the enzyme reaction takes place in adiabatic cell. Constant temperature of the thermostat was realized using thermoelectrics.Constant temperature effect of the system is very good; the enzyme reaction heat can be detected by flow injection analysis andthe application of this system in pesticide detection can be validated.

关 键 词：酶法 农药残留量 量热系统 小麦酯酶 流动注射分析 检测技术 

分 类 号：TS207.53[轻工技术与工程—食品科学]