固定化“双功能”酵母基因工程菌在酒精工业生产中的应用  

Application of Immobilized " Double Functions " Genetic Engineering Yeast in the Production of Alcohol

作　　者：吴淑魁 罗进贤[2] 陈海滨 李文清[2] 叶若邻[2] 黄树华 程少菊[2] 周玲 

机构地区：[1]广西柳州市京龙科技开发有限公司,广西柳州545006 [2]中山大学生命科学院,广东广州510275

出　　处：《酿酒科技》2004年第2期61-62,64,共3页Liquor-Making Science & Technology

基　　金：广东省自然科学基金(940628);广州市重点科技项目(96-226-2);广西柳州市科技开发计划(20020106)

摘　　要：应用基因克隆技术将黑曲霉产糖化酶基因cDNA转入经优化的受体菌(酿酒酵母京龙JL108号),获得多株具有产糖化酶能力的酿酒酵母,再经反复筛分、驯化获得JL1(YIp128D.17N),采用变性PVA固定化增殖活细胞包埋技术,将JL1制成具有糖化酒化“双功能”的固定化酵母,大幅度提高细胞数,增强了大生产基因工程菌的糖化、酒化能力,载体产酶能力在10u/g·h以上,酒精发酵醪液中酶活达20u/ml以上,在年产5000t的富川县酒精厂进行工业性生产应用试验,技术指标完全达到并超过传统工艺的生产水平,淀粉出酒率达55%以上。The gene cDNA of aspergillus niger(wh ic h produced saccharifying enzyme)was transferred into the optimized acceptor yea st(liquor-making yeast Jinglong JL108)by genic clone techniques.As a resu lt,multiple strains of liquor-making yeast in posses-sion of saccharifying en zyme-producing capability were obtained.After repetitive selection and domesti cation,the strain of JL1(YIp128D.17N)was obtained.Then JL1was made i nto immobilized yeast in possession of'double functions'including saccharifyin g capability and alcoholizing capability by the technique of denaturing PVA immo bilized proliferation living cell embedment.The tech-nique could greatly impro ve cell amount and strengthen saccharifying capability and alcoholizing capabili ty of genetic engineering yeast in mass production.The enzyme-producing capabi lity of the carrier was above10u/g·h and the enzyme survivability in ferment ed liq-uid was above20u/ml.The trial production in Fuchuan Grain Distillery with annual alcohol yield as5,000tons proved that the tech-nical indexes had completely achieved or exceeded the corresponding indexes before.And the yi eld rate of amylum was above55%.(Tran.by YUE Yang)

关 键 词：基因工程 双功能菌 固定化 糖化酶基因 酵母基因工程菌 

分 类 号：TS261[轻工技术与工程—发酵工程]