用于固定化米根霉发酵生产L-乳酸的转盘反应器及其放大  

A Rotating Disc Contactor used for L-lactic acid production by immobilized Rhizopus oryzae and its scale-up

作　　者：张定丰[1] 林建平[2] 金志华[2] 岑沛霖[2] 

机构地区：[1]浙江医药股份有限公司新昌制药厂,浙江新昌312500 [2]浙江大学材料与化工学院,浙江杭州310027

出　　处：《化学工程》2004年第1期34-37,共4页Chemical Engineering(China)

摘　　要：利用米根霉菌丝体发达且易于吸附在固体表面的特性,将其固定化于转盘表面,利用转盘反应器实现L 乳酸的固定化发酵。考察了固定化过程中的底物浓度、产物浓度和溶解氧浓度的变化规律。在此基础上,进一步研究了8L反应器的通气量、装液量及转盘转速等操作参数对发酵过程的影响,得到了优化的反应器操作条件。最后,利用自制的1m3反应器考察了转盘反应器固定化发酵的可行性,得到了比较满意的结果,证实该反应器具有良好的工业应用前景。Based on its well-developed mycelium and well adhering to solid surface, Rhizopus oryzae was immobileized on the surface of rotating discs in a bioreactor called Rotating Disc Contactor (RDC) to conduct L-lactic acid production. The time courses of substrate consumption, product formation and dissolved oxygen concentration during the immobilization process were measured, which indicated that the spore germination consumes oxygen quickly. The effects of aeration rate, volume of broth and rotating rate of discs on the productivity and yield of L-lactic acid were studied in an 8 L RDC. And the results indicate that if aeration rate is higher than 0.075 m^3/h, the L-lactic acid productivity and conversion ratio would be maintained almost constant. The RDC was successfully scaled up to 1 m^3. The results show that RDC can be used for repeated-batch fermentation of L-lactic acid.

关 键 词：米根霉 固定化 发酵 L-乳酸 转盘反应器 

分 类 号：TQ920[轻工技术与工程—发酵工程]