无溶剂体系合成1,3-甘油二酯用脂肪酶的筛选及其酯化性质  

Screening of Lipase for Synthesis of 1,3-sn-Diacylglycerols and Its Esterification Characteristics in Solvent-Free System

作　　者：孟祥河[1] 邹冬芽[1] 段作营[1] 毛忠贵[1] 

机构地区：[1]江南大学生物工程学院教育部生物技术重点实验室,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第2期31-35,共5页Journal of Wuxi University of Light Industry

摘　　要：对不同来源的脂肪酶进行了系统的筛选,并根据位置专一性的差别将脂肪酶分为1,3 特异的、1,3 选择性和非选择性的3类.采用LipozymeRMIM在无溶剂系统中催化甘油、脂肪酸合成1,3 sn 甘油二酯.研究表明脂肪酸、底物醇种类、温度、水分活度对LipozymeRMIM的活力有明显的影响.Lipases from different commercial sources were screened in terms of their regioselectivities (RE) and activity of esterification. Obvious differences were found in the RE value so that it can be selected as a measure to classify these biocatalysts as those of specific, selective, and nonspecific. On the base of systematic screening experiments, Lipozyme RM^(IM) indicating high regioselectivites and high esterification activity was used as the catalyst for efficient synthesis of 1,3-sn-Diglyceride with glycerol and fatty acid as the raw materials in solvent-free system . The results suggested that the species of fatty acids and alcohols, reaction temperature, and water activity effected esterification activity of Lipozyme RM^(IM) significantly.

关 键 词：脂肪酶 1 3-甘油二酯 筛选 甘油 脂肪酸 催化合成 酯化特性 LIPOZYME RM^IM 

分 类 号：Q55[生物学—生物化学]