薄层色谱法和柱层析法分离兰州红心萝卜色素的研究  

Study on Pigments Extraction from Lanzhou Red Radish by Thin-Layer Chromatography and Column

作　　者：傅正生[1] 薛华丽[1] 王长青[1] 刘茂栋[1] 马景毅[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《食品科学》2004年第6期49-52,共4页Food Science

基　　金：甘肃省教育厅自然科学基金资助项目(011-01)

摘　　要：利用薄层色谱法和柱层析法对红心萝卜中的色素进行分离分析,着重研究了色素的量、洗脱剂、洗脱时间等因素对色素分离的影响。选择了以硅胶G为固定相,无水乙醇/水(5:1,V/V)的混合液作洗脱剂的最佳条件,单向一次性进行色谱分离。In order to extracting and analyzing red radish pigments, the thin-layer chromatography and column were usedto study the optimum factors such as the quantity of pigment, developing agent, developing time etc. That would affect the pigmentsextraction. The experiment showed that both the silica gel G used as the fixed phase and the mixture solution ratio of anhydrousalcohol and water at (5:1, V/V) were the optimum conditions.

关 键 词：薄层色谱法 柱层析法 红心萝卜 色素 分离 洗脱剂 

分 类 号：TS202.3[轻工技术与工程—食品科学]