The Primary Motor Cortex Stimulation Attenuates Cold Allodynia in a Chronic Peripheral Neuropathic Pain Condition in <i>Rattus norvegicus</i>  

作　　者：Priscila Medeiros Sylmara Esther Negrini-Ferrari Ana Carolina Medeiros Lais Leite Ferreira Josie Resende Torres da Silva José Aparecido da Silva Norberto Cysne Coimbra Renato Leonardo de Freitas 

机构地区：[1]Neurosciences aboratory of Pain & Emotions and Multi-User Centre of Neuroelectrophysiology, Department of Surgery and Anatomy, Ribeirã o Preto Medical School of the University of Sã o Paulo, Ribeirã o Preto, Sã o Paulo, Brazil [2]Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirã o Preto Medical School of the University of Sã o Paulo (FMRP-USP), Ribeirã o Preto (SP), Brazil [3]Biomedical Sciences Institute, Federal University of Alfenas (UNIFAL-MG), Alfenas, Minas Gerais (MG), Brazil [4]Motricity Sciences Institute, UNIFAL-MG, Alfenas, MG, Brazil [5]Post-Graduate Program of Biosciences Applied to Health (PPGB), UNIFAL, Biomedical Sciences Institute, Alfenas, MG, Brazil [6]Department of Psychology, Ribeirã o Preto School of Philosophy, Sciences and Literature of the University of Sã o Paulo FFCLRP-USP, Ribeirã o Preto, Brazil [7]Department of Psychology, Federal University of Juiz de Fora (UFJF-MG), Juiz de Fora (MG), Brazil [8]Behavioural Neurosciences Institute (INeC), Ribeirã o Preto (SP), Brazil

出　　处：《World Journal of Neuroscience》2019年第3期138-152,共15页神经科学国际期刊（英文）

摘　　要：Background: The primary motor cortex (M1) stimulation (MCS) is a useful tool for attenuation of the peripheral neuropathic pain in patients with pharmacologically refractory pain. Furthermore, that neurological procedure may also cause antinociception in rodents with neuropathic pain. Cold allodynia is a frequent clinical finding in patients with neuropathic pain, then, we evaluated if an adapted model of neuropathy induced by chronic constriction injury (CCI) of the ischiadicus nervus (sciatic nerve) produces cold allodynia in an animal model of chronic pain. In addition, we also investigated the effect of the electrical stimulation of the M1 on chronic neuropathic pain condition in laboratory animals. Methods: Male Wistar rats were used. An adapted model of peripheral mononeuropathy induced by CCI was carried out by placing a single loose ligature around the right sciatic nerve. The acetone test was used to evaluate the cold allodynia in CCI or Sham (without ligature) rats. The MCS (M1) was performed at low-frequency (20 μA, 100 Hz) during 15 s by deep brain stimulation (DBS-Thomas Recording device) 21 days after CCI or Sham procedures. The cold allodynia was measured before and immediately after the neurostimulation of M1 in the following time-window: 0, 15 and 30 min after MCS. Results: Cold allodynia threshold increased in animals with chronic neuropathic pain submitted to the acetone test 21 days after the CCI surgery. The M1-stimulation by DBS procedure decreased the cold allodynia immediately and until 30 min after M1-stimulation in rats with chronic neuropathic pain. Conclusion: The current proposal for a CCI model by a single loose ligature of the sciatic nerve can be employed as an experimental model of chronic neuropathic pain in rats submitted to peripheral nervous system injury. The M1-stimulation produced antinociception in rats with chronic neuropathic pain. Thus, we reinforced that the MCS decreases cold allodynia in laboratory animals submitted to persistent sciatic nerve constriction and can bBackground: The primary motor cortex (M1) stimulation (MCS) is a useful tool for attenuation of the peripheral neuropathic pain in patients with pharmacologically refractory pain. Furthermore, that neurological procedure may also cause antinociception in rodents with neuropathic pain. Cold allodynia is a frequent clinical finding in patients with neuropathic pain, then, we evaluated if an adapted model of neuropathy induced by chronic constriction injury (CCI) of the ischiadicus nervus (sciatic nerve) produces cold allodynia in an animal model of chronic pain. In addition, we also investigated the effect of the electrical stimulation of the M1 on chronic neuropathic pain condition in laboratory animals. Methods: Male Wistar rats were used. An adapted model of peripheral mononeuropathy induced by CCI was carried out by placing a single loose ligature around the right sciatic nerve. The acetone test was used to evaluate the cold allodynia in CCI or Sham (without ligature) rats. The MCS (M1) was performed at low-frequency (20 μA, 100 Hz) during 15 s by deep brain stimulation (DBS-Thomas Recording device) 21 days after CCI or Sham procedures. The cold allodynia was measured before and immediately after the neurostimulation of M1 in the following time-window: 0, 15 and 30 min after MCS. Results: Cold allodynia threshold increased in animals with chronic neuropathic pain submitted to the acetone test 21 days after the CCI surgery. The M1-stimulation by DBS procedure decreased the cold allodynia immediately and until 30 min after M1-stimulation in rats with chronic neuropathic pain. Conclusion: The current proposal for a CCI model by a single loose ligature of the sciatic nerve can be employed as an experimental model of chronic neuropathic pain in rats submitted to peripheral nervous system injury. The M1-stimulation produced antinociception in rats with chronic neuropathic pain. Thus, we reinforced that the MCS decreases cold allodynia in laboratory animals submitted to persistent sciatic nerve constriction and can b

关 键 词：Peripheral Neuropathic PAIN CHRONIC PAIN Sciatic Nerve CHRONIC Constriction Injury Cold ALLODYNIA Primary Motor Cortex STIMULATION ANTINOCICEPTION 

分 类 号：R74[医药卫生—神经病学与精神病学]