Improved Solid-Phase Peptide Synthesis of Wild-Type and Phosphorylated Phospholamban Using a Pseudoproline Dipeptide  

作　　者：Shadi Abu-Baker Gary A. Lorigan 

机构地区：[1]Department of Chemistry and Biochemistry, Miami University, Oxford, USA

出　　处：《Open Journal of Synthesis Theory and Applications》2012年第2期9-12,共4页合成理论与应用（英文）

摘　　要：In this study, we report that the insertion of a pseudoproline dipeptide for the solid-phase peptide synthesis of wild-type Phospholamban protein (WT-PLB) has two important advantages. First, it disrupts the formation of different secondary structures, which is responsible for poor couplings during the preparation of highly aggregated sequences. Second, it enhances the purities and solubility of crude products leading to easier HPLC purification.In this study, we report that the insertion of a pseudoproline dipeptide for the solid-phase peptide synthesis of wild-type Phospholamban protein (WT-PLB) has two important advantages. First, it disrupts the formation of different secondary structures, which is responsible for poor couplings during the preparation of highly aggregated sequences. Second, it enhances the purities and solubility of crude products leading to easier HPLC purification.

关 键 词：SOLID-STATE PEPTIDE SYNTHESIS Pseudoproline DIPEPTIDE PHOSPHOLAMBAN 

分 类 号：R73[医药卫生—肿瘤]