国家自然科学基金(30570405)

作品数：4被引量：5H指数：2
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Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E. coil
《Chemical Research in Chinese Universities》2008年第6期752-755,共4页WANG Rui-jian YANG Xue-ying ZHENG Liang-yu YANG Ye GAO Gui CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos. 20432010, 20672045 and 30570405)
The entire gene of carboxyltransferase（CT） domain of acetyl-CoA carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed...
关键词：Chinese Spring wheat Acetyl-CoA carboxylase Carboxyltransferase（CT） domain CLONING Expression PURIFICATION 
High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate被引量：1
《Chemical Research in Chinese Universities》2007年第3期319-324,共6页ZHANG Gui-rong GAO Ren-jun ZHANG Ai-jun RAO Lang CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos30400081, 30570405 and 20672045);the Key Tech-nology Research and Development Program of China(No2004BA713D03-04)
To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a ...
关键词：High-throughput screening ENANTIOSELECTIVITY Hyperthermophilic esterase Directed evolution 
Expression and Purification of Active Recombinant Human Nerve Growth Factor from Escherichia coli被引量：3
《Chemical Research in Chinese Universities》2007年第2期237-240,共4页HE Ling-bing WANG Yan WANG Ge CHEN Chao CAO Shu-gui 
the National Natural Science Foundation of China(Nos 20432010, 30570405 and 20672045)
Introduction Nerve growth factor （NGF） was first discovered and purified by Rita Levi-Montalcini and Stanley Cohen in the 1950s. It represents the first cellular growth factor ever discovered and involved in the gl...
关键词：Nerve growth factor Fusion protein PURIFICATION Biological activity 
Denaturing Effects of Urea and Guanidine Hydrochloride on Hyperthermophilic Esterase from Aeropyrum pernix K1被引量：2
《Chemical Research in Chinese Universities》2006年第2期168-172,共5页GAO Ren-jun XIE Gui-qiu ZHOU Jun FENG Yan CAO Shu-gui 
SupportedbytheNationalNaturalScienceFoundationofChina(Nos.30400081,20272017and30570405)andtheKeyTechnologiesResearchandDevelopmentProgrammer(No.2001BA708B0306).
The changes in the activity and the conformation of the hyperthermophilic esterase derived from aerobic thermophilic Aeropyrum pernix K1 （ APE1547 ） were studied during denaturation by guanidine hydrochlofide （ Gdn...
关键词：STABILITY Hyperthermophilic esterase UREA Guanidine hydrochloride 
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