supported by the State Key Basic Research and Development Plan of China(Grant No.001CB1089-06);the National Science Foundation for Distinguished Young Scholars of China(Grant No.20403024);the National Natural Science Foundation of China(Grant No.30270296).
supported by the State Key Basic Research and Developmental Plan of China(Grant No.001CB1089-06);the National Natural Science Foundation of China(Grant No.30270296);the National Science Foundation for Distin-guished Young Scholars of China(Grant No.20403024).
A MoFe protein (ΔnifE Av1) with a purity of ~80% was purified from a nifE-deleted mutant of Azotobacter vinelandii DJ35. Compared with MoFe protein purified from wild-type strain OP (OP Av1), ΔnifE Av1 had the same...
supported by the State Key Basic Research and Developmental Plan of China(Grant No.001CB1089-06);the National Natural Science Foundation of China(Grant No.30270296);the National Science Foundation for distinguished Young Scholars of China(Grant No.20403024).
Through the anaerobic chromatography on the columns of DEAE 52, Q-Sepharose and Sephacryl S-200, a nitrogenase MoFe protein (?nifZ Av1) was obtained from a nifZ deleted mutant of Azotobacter vinelandii (stain DJ194). ...
Nitrogenase CrFe protein and MnFe protein were purified from a mutant strain UW3 of Azotobacter vinelandii Lipmann grown on a medium containing Cr and Mn, respectively. In order to meet the requirement for crystal gro...
Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from FeMoco-deficient MoFe protein (DeltanifE Avl) purified from a nifE deleted mutant DJ35 of Azotobacter vinela...
By using the liquid/liquid diffusion method at a suitable crystallization conditions, large single and dark brown crystals (the sides of the largest crystals were 0.20 mm x 0.20 mm x 0.07 min and 0.18 mm x 0.18 mm x 0...