This work was supported by the National Natural Science Foundation of China(No.20443005);the Nanotechnology Special Projects of Shanghai(No.0352nm123)
A novel artificial site specific cleavage reagent, with peptide nucleic acid (PNA) as sequence-recognizing moiety and cerium (IV) ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was...
supported by the National Natural Science Foundation of China(Grant No.20443005);the Nanotechnology Special Projects of Shanghai(Grant No.0352nm123);the Major Project Foundation of Shanghai Education Administration(Grant No.04DA01).
Polymerase chain reaction (PCR) is a useful technique for in vitro amplification of a DNA fragment. In this paper, a PCR procedure using Au nanoparticle (AuNP) -bound primers was systemically studied. The 5′-SH- (CH2...