supported by the National Natural Science Foundation of China (60025516 and 10334100);the Scientific Research Start-up Foundation of China Agricultural University (2009-2-05)
Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, DNA-histone complexes are stretched on a hydrophobic polymethyl ...
supported by the National Natural Science Foundation of China(60025516 and 10334100);the Scientific Research Startup Foundation of China Agricultural University(2009-2-05)
The binding of cations (Na +,K +,Mg 2+,Ca 2+,Mn 2+) and histones to DNA can be studied using fluorescence assays.Here,we measured the fluorescence intensity and fluorescence anisotropy of DNA and DNA-histone complexes...
Supported by the National Natural Science Foundation ofChina ( No . 60025516 , No . 10334100 , and No .60138010 );the Innovation Project of the ChineseAcademy of Sciences ,K.C.Wong Post-doctoral ResearchAward ( No .40 );partly supported by NationalCenter for Nanoscience and Technology
This work was supported by the grants from the National Natural Science Foundation of China (No.60025516 and No.10334100);the Innovation Project of the Chinese Academy of Sciences,and the Centre National de la Recherche Scientifique (CNRS)
A fluorometric assay was used to study the DNA unwinding kinetics induced by Escherichia coli RecQ helicase. This assay was based on fluorescence resonance energy transfer and carried out on stopped-flow, in which DNA...