国家自然科学基金(20733001)

作品数：8被引量：4H指数：1
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相关作者：赵新生尹延东支泽勇周晓雪刘鹏程更多>>
相关机构：北京大学更多>>
相关期刊：《物理化学学报》《Science China Chemistry》《Chinese Journal of Chemistry》《高等学校化学学报》更多>>
相关主题：单分子探测单分子英文MAMMALIAN_CELLSINTEGRATED更多>>
相关领域：理学生物学电子电信更多>>
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Rebinding kinetics of dissociated amino acid ligand and carbon monoxide to ferrous microperoxidase-11 in aqueous solution
《Science China Chemistry》2013年第2期230-237,共8页ZHONG RangBo LU Rong YU AnChi 
financially supported by the National Natural Science Foundation of China (20603047, 20733001);the Fundamental Research Funds for the Central Universities;the Research Funds of Renmin University of China (10XNJ047)
The rebinding kinetics of an amino acid ligand to ferrous microperoxidase-11 (MP11) after photolysis of aggregated ferrous MP11 was measured in aqueous solution with femtosecond transient visible absorption spectrosco...
关键词：femtosecond transient absorption spectroscopy ferrous microperoxidase-11 concentration-dependent recombination kinetics aggregation tendency carbon monoxide 
An integrated microfluidic device for long-term culture of isolated single mammalian cells被引量：2
《Science China Chemistry》2012年第4期502-507,共6页ZHENG ChunHong CHEN Gui’E PANG YuHong HUANG YanYi 
supported by the National Natural Science Foundation of China (20733001, 20890020, 90913011, 20905004);the Ministry of Science and Technology of China (2011CB809106);the Ministry of Education of China;the Fok Ying Tung Education Foundation
We developed an integrated microfluidic chip for long-term culture of isolated single cells. This polydimethylsiloxane (PDMS) based device could accurately seed each single cell into different culture chambers, and is...
关键词：MICROFLUIDICS single cell culture conditioned medium 
Cation and anion substitution effects on the ultrafast dynamics of interionic interaction in imidazolium based ionic liquids
《Science China Chemistry》2011年第9期1491-1497,共7页LU Rong WANG Wei YU AnChi 
supported by the National Natural Science Foundation of China (20733001,20803092);the Fundamental Research Funds for the Central Universities;the Research Funds of Renmin University of China (10XNI007,10XNJ008)
Room-temperature Ionic Liquids(ILs) have numerous unique properties that differ from those of conventional molecular solvents.Although the unique properties of ILs have been suggested to origin from their microscopic ...
关键词：Brownian oscillator ultrafast dynamics interionic interaction ionic liquid Raman induced Kerr effect spectroscopy 
用于单分子动力学实验的微流控混合器(英文)被引量：1
《物理化学学报》2011年第8期1990-1995,共6页支泽勇 刘鹏程 黄岩谊 赵新生 
supported by the National Natural Science Foundation of China(20733001,20973015);National Key Basic Research Program of China(973)(2006CB910300,2010CB912302)~~
设计制作了用于单分子动力学实验的微流控混合器,该混合器用聚二甲基硅氧烷(PDMS)芯片和石英载玻片密封而成,具有低的荧光背景,广泛的生物相容性,结合激光共聚焦显微镜能够在非平衡态下进行单分子荧光探测.我们设计的压力控制系统和进...
关键词：微流控混合 单分子探测 荧光共振能量传递 蛋白质折叠 金黄色葡萄球菌核酸酶 
Aggregation Behaviors of Tricarbocyanine Dye in Water and in AOT Reverse Micelles
《Chinese Journal of Chemistry》2011年第3期405-410,共6页Zhu, Ruixue Lu, Rong Yu, Anchi 
Project supported by the National Natural Science Foundation of China （Nos. 20603047, 20733001） and Renmin University of China.
The photophysical property of the tricarbocyanine dye IR144 has been extensively studied in non-aqueous solvents. However, as a potential near-infrared biomedical imaging probe, the photophysical property of IR144 in ...
关键词：PHOTOPHYSICS PHOTOCHEMISTRY KINETICS IR 144 AGGREGATES AOT reverse micelles 
最大熵法分析寡聚核苷酸链内碰撞的荧光相关光谱(英文)被引量：1
《物理化学学报》2010年第4期1087-1092,共6页尹延东 周晓雪 赵新生 
supported by the National Natural Science Foundation of Chinca(20673002,20733001,20973015);National Key Basic Research Program of China(973)(2006CB910304,2010CB912302)~~
准确地由荧光相关光谱(FCS)的实验数据提取动力学信息一直是一个挑战.本文对比了三种主要的方法:依赖于模型的多指数函数法,经验的拓展指数函数法和不依赖于模型的最大熵法.多指数函数法的物理意义直接但在复杂体系中难以应用和解释.拓...
关键词：荧光相关光谱 弛豫时间分布 拓展指数函数 最大熵法 寡聚核苷酸 光致电子转移 
Fluorescence quenching of TMR by guanosine in oligonucleotides被引量：1
《Science China Chemistry》2009年第10期1653-1659,共7页QU Peng CHEN XuDong ZHOU XiaoXue LI Xun ZHAO XinSheng 
Supported by the National Natural Science Foundation of China (Grant Nos. 20673002, 20733001);the 973 Project (2006CB910304)
Nucleotide-specific fluorescence quenching in fluorescently labeled DNA has many applications in biotechnology. We have studied the inter-and intra-molecular quenching of tetramethylrhodamine (TMR) by nucleotides to b...
关键词：TMR GUANOSINE FLUORESCENCE OLIGONUCLEOTIDES G-quenching PET FCS 
光学单分子方法探测蛋白质的功能(英文)
《高等学校化学学报》2008年第12期2635-2642,共8页李勋 赵新生 
国家重点基础研究发展计划(批准号:2006CB910304);国家自然科学基金(批准号:20673002,20733001)资助
过去20年中,光学单分子探测的方法已经发展成为许多领域、特别是生物学领域研究的强有力工具.单分子探测不仅能够给出物理量的平均值,还可以给出有关分布的信息,也能对一个分子的轨迹进行追踪.这些独特的性质使单分子探测不仅仅在平衡体...
关键词：单分子探测 蛋白质 动力学 
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