云南高校图书馆联盟文献共享服务平台- 国家重点基础研究发展计划(2007CB714507)

国家重点基础研究发展计划(2007CB714507): 作品数：13被引量：49H指数：4; 导出分析报告; 相关作者：梁淑轩林金明辛天兵靳辉何治柯更多>>; 相关机构：武汉大学北京科美东雅生物技术有限公司清华大学河北大学更多>>; 相关期刊：《Science China Chemistry》《分析化学》《Science Bulletin》《科学通报》更多>>; 相关主题：CHEMILUMINESCENCE人血清MAGNETIC化学发光微流控芯片更多>>; 相关领域：理学医药卫生生物学轻工技术与工程更多>>

Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein被引量：5: 《Journal of Pharmaceutical Analysis》2012年第2期130-135,共6页Qian-Yun Zhang a,b,Hui Chen a,Zhen Lin a,Jin-Ming Lin a a Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Department of Chemistry,Tsinghua University,Beijing 100029,China b Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China; supported by the National Basic Research Program of China (973 Program,no. 2007CB714507);National Nature Science Foundation of China (no. 90813015); A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELI...; 关键词：a-Fetoprotein Hepatocellular carcinoma Chemiluminescence enzyme immunoassay Magnetic microparticles Colorimetric enzyme-linked immunosorbent assay

Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid被引量：4: 《Science China Chemistry》2011年第8期1298-1303,共6页ZHOU GuoHua, WANG Ping, YUAN Ju, QIU Ting & HE ZhiKe Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China; supported by the National Natural Science Foundation of China (21075093 & 90717111);the Science Fund for Creative Research Groups of the National Natural Science Foundation of China (20921062);the National Key Scientific Program-Nanoscience and Nanotechnology (2011CB933600);the National Basic Research Program of China (2007CB714507); Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical ...; 关键词：abscisic acid immunomagnetic assay CHEMILUMINESCENCE CDSE/ZNS

Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatocellular carcinoma marker glypican-3被引量：8: 《Journal of Pharmaceutical Analysis》2011年第3期166-174,共9页Qian-Yun Zhang Hui Chen Zhen Lin Jin-Ming Lin; supported by the National Basic Research Program of China (973 Program,No.2007CB714507);the National Natural Science Foundation of China (No.90813015); Glypican-3 （GPC3） is reported as a great promising tumor marker for hepatocellular carcinoma （HCC） diagnosis. Highly sensitive and accurate analysis of serum GPC3 （sGPC3）, in combination with or instead of tradi...; 关键词：Magnetic nanoparticle Magnetic microparticleChemiluminescenceenzyme immunoassay GLYPICAN-3 Hepatocellularcarcinoma

纸芯片制作及其在化学发光法检测葡萄糖和尿酸中的应用被引量：11: 《分析科学学报》2011年第2期137-141,共5页王方方陈锦何治柯; 国家973计划(No.2007CB714507); 纸芯片是一类新型的个性化诊断器件,可快速、廉价、简便地进行多组分分析。采用光刻法制备了一维和二维纸芯片(μPADs),并对不同通道芯片检测所需的样品体积进行理论估算。同时利用葡萄糖氧化酶-葡萄糖反应产生过氧化氢诱导鲁米诺化学发...; 关键词：纸芯片化学发光葡萄糖尿酸

Chemiluminescence behavior of sodium hydrogen carbonate in the potassium permanganate-hydrogen peroxide reaction被引量：3: 《Science China Chemistry》2010年第8期1784-1792,共9页CHEN Hui1,2,LU Chao1,LI RuiBo1,GUO GuangSheng1 & LIN Jin-Ming2 1State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology,Beijing 100029,China 2Department of Chemistry,Tsinghua University,Beijing 100084,China; support from the National Natural Science Foundation of China (209350002);the National Basic Research Program of China (2007CB714507); Chemiluminescence (CL) phenomenon of hydrogen peroxide with potassium permanganate in the presence of sodium hydrogen carbonate was reported.Effects of the surfactant on the CL system were investigated.Nonionic surfac...; 关键词：CHEMILUMINESCENCE POTASSIUM PERMANGANATE EMITTING species energy transfer

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection: 《Journal of Pharmaceutical Analysis》2010年第2期97-101,共5页Qin Xiao1,2,Hui Chen2,Jin-Ming Lin2 1. College of Ocean,Hebei Agricultural University,Qinhuangdao 066003 2. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China.; supported by the National Key Technology R & D Program (2006BAK02A13);the National Basic Research Program of China (973Program, No.2007CB714507); A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET...; 关键词：ATP bioluminescence bacterial detection EXPRESSION firefly luciferase PURIFICATION

A microchip to analyze single crystal growth and size-controllability被引量：1: 《Science China Chemistry》2009年第7期1014-1020,共7页PANG YuanFeng LIU JiangJiang LI HaiFang LIN JinMing; Supported by the National Natural Science Foundation of China (Grant No. 20775042);the National Basic Research Program of China (Grant No. 2007CB714507); A microfluidic device to control single crystallization on the micron scale has been developed. The salt solution was stored in the nano-volume gaps between the arrays of protrudent circular plots in the microchip. Th...; 关键词：microfluidic device single CRYSTALLIZATION LIQUID-LIQUID interface size CONTROLLABILITY

微流控芯片上镍带对磁珠运动方向的控制以及磁响应不对称现象: 《分析化学》2009年第A03期270-270,共1页余旭张志凌庞代文; 本文系973计划项目（No.2007CB714507）资助; 微流控芯片是一种将各种生化分析功能集中到一块厘米见方的芯片上。在微流控芯片上结合磁珠技术的优势一直都是研究的热点之一。然而，在只有几十到数百微米的微流体通道内对磁珠的进行控制一直都是比较困难的问题。常见方法有制作微型...; 关键词：微流控芯片不对称现象磁珠运动方向控制磁响应镍流体通道

微流控芯片中液滴断裂：由皮升级到飞升级被引量：1: 《分析化学》2009年第A03期271-271,共1页冯璇张志凌庞代文; 本文系973计划项目（No.2007CB714507）资助; 近年来，微流控芯片中形成液滴备受关注。由于在微流控芯片中形成的液滴具有大的比表面积，传质传热时间，扩散距离，反应时间明显缩短，产生的液滴体积小，大大降低了样品的消耗量，节约了实验成本，在短时间内可以形成大量液滴，为进...; 关键词：微流控芯片液滴断裂传热时间比表面积扩散距离反应时间实验成本

毛细管电泳化学发光检测系统新进展被引量：3: 《分析化学》2008年第11期1579-1586,共8页吉邢虎徐秦峰何治柯; 教育部博士点基金(No.20050486026);973计划(No.2007CB714507)资助; 分析技术的微型化、集成化和自动化是当前分析化学发展的一个新趋势。本文综述了毛细管电泳化学发光检测系统微型化、集成化和自动化的最新研究进展。; 关键词：毛细管电泳化学发光检测微型化集成化自动化

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