云南高校图书馆联盟文献共享服务平台- 国家重点基础研究发展计划(2010CB126203)

国家重点基础研究发展计划(2010CB126203): 作品数：10被引量：51H指数：4; 导出分析报告; 相关作者：周益军卢嫣红陈剑平张金凤徐秋芳更多>>; 相关机构：江苏省农业科学院南京农业大学浙江省农业科学院浙江师范大学更多>>; 相关期刊：《中国科学：生命科学》《Science China(Life Sciences)》《中国农业科学》《Journal of Integrative Agriculture》更多>>; 相关主题：RICE南方水稻黑条矮缩病毒水稻黑条矮缩病毒DWARF抑制子更多>>; 相关领域：农业科学生物学环境科学与工程更多>>

Highly Sensitive and Specific Monoclonal Antibody-Based Serological Methods for Rice Ragged Stunt Virus Detection in Rice Plants and Rice Brown Planthopper Vectors被引量：5: 《Journal of Integrative Agriculture》2014年第9期1943-1951,共9页LIU Huan SONG Xi-jiao NI Yue-qun LU Li-na ZHOU Xue-ping WU Jian-xiang; supported by the National Basic Research Program of China(2010CB126203);the special fund for Agro-scientific Research in the Public Interest,China(201003031);Earmarked Funds for Modern Agro-industry Technology Research System;Zhejiang Provincial Natural Science Foundation of China(Z3090039); Rice ragged stunt virus（RRSV） is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein（CP） gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21（...; 关键词：Rice ragged stunt virus rice brown planthopper monoclonal antibody antigen-coated-plate enzyme-linked immunosorbent assay（ACP-ELISA） dot-blot ELISA immunocapture RT-PCR

水稻黑条矮缩病毒P9-1在烟草表皮细胞中自我互作并形成包涵体: 《浙江农业学报》2014年第2期365-370,共6页谭子龙孙丽英陈剑平; 973项目(2010CB126203);国际科技合作计划(2012DFA30900);国家自然科学基金(31071660);浙江省植物病毒与组培产业重点领域创新团队(2009R50032); 水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)是斐济病毒属的成员,由昆虫介体灰飞虱传播,能够在植物宿主和昆虫介体中复制。同其他斐济病毒成员一样,RBSDV的复制与装配是在细胞质病毒包涵体结构即毒质结构里进行的。RBSDV...; 关键词：水稻黑条矮缩病毒包涵体自我互作

Detection,Occurrence,and Survey of Rice Stripe and BlackStreaked Dwarf Diseases in Zhejiang Province,China被引量：4: 《Rice science》2013年第6期383-390,共8页ZHANG Heng-mu WANG Hua-di YANG Jian Michael J ADAMS CHEN Jian-ping; funded by the National Science and Technology Support Program of China (Grant No. 2012BAD19B03);the China 863 and 973 Program (Grant Nos. 2007AA10Z414 and 2010CB126203);the International Science and Technology Cooperation Project (Grant No. 2007DFB30350);the Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003031);the Zhejiang Provincial Science and Technology Project (Grant No. 2010C12027);the Zhejiang Provincial Foundation for Natural Science (Grant Nos. Z305165 and Y3090657); The major viral diseases that occur on rice plants in Zhejiang Province, eastern China, are stripe and rice black-streaked dwarf diseases. Rice stripe disease is only caused by rice stripe tenuivirus （RSV）, while ri...; 关键词：viral diseases rice stripe tenuivirus rice black-streaked dwarf fijivirus southern rice black-streaked dwarf ftjivirus surveys

Screening of Rice Genes Interacting with p5b of Rice BlackStreaked Dwarf Virus: 《Rice science》2013年第3期185-190,共6页LU Ying YANG Jian ZHANG Heng-mu CHEN Jian-ping; funded by the National Science and Technology Support Program of China(Grant No.2012BAD19B03);the National High Technology Research and Development Program of China(Grant No.2007AA10Z414);the National Basic Research Program of China(Grant No.2010CB126203);the International Science and Technology Cooperation Project(Grant No.2007DFB30350);the Special Fund for Agro-scientific Research in the Public Interest of China(Grant No.201003031);the Zhejiang Provincial Science and Technology Project,China(Grant No.2010C12027);the Zhejiang Provincial Foundation for Natural Science,China(Grant Nos.Z305165and Y3090657); Rice black-streaked dwarf virus （RBSDV） is a recognized member of the genus Fijivirus, family Reoviridae. Its genome has ten double-stranded RNA （dsRNA） segments （$1-$10）, in which the fifth genome segment （$5...; 关键词：rice black-streaked dwarf virus interaction protein yeast two-hybrid

南方水稻黑条矮缩病毒SP7-1植物表达载体的构建及遗传转化被引量：1: 《江苏农业学报》2012年第6期1278-1282,共5页苑侠孙枫卢嫣红周彤范永坚周益军; 国家公益性行业(农业)科研专项(201003031);江苏省自然科学基金(BK2010018);国家"973"计划(2010CB126203);江苏省农业科技自主创新基金项目[CX(10)207]; 克隆南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)的SP7-1基因,构建植物表达载体并转化拟南芥,为研究SP7-1致病机理提供基础。根据已发表的SRBSDV SP7-1序列,设计特异性引物,通过RT-PCR的方法从感病水稻植...; 关键词：南方水稻黑条矮缩病毒 SP7-1基因拟南芥转化

水稻黑条矮缩病毒基因组S7编码的2个非结构蛋白在病株中的表达检测被引量：7: 《中国水稻科学》2012年第1期9-15,共7页吕明芳羊健张恒木陈剑平; 国家973计划资助项目(2010CB126203);浙江省自然科学基金资助项目(Y3090657); 水稻黑条矮缩病毒(RBSDV)基因组片段S7含有2个非重叠的阅读框,所编码的蛋白分别为p7a和p7b。根据RBSDV S7序列(EU111804)设计特异性引物分别扩增编码p7a和p7b蛋白的基因片段,并亚克隆至原核表达载体pET-32a(+)和pSBET上,再以大肠杆菌BL2...; 关键词：水稻黑条矮缩病毒非结构蛋白原核表达

南方水稻黑条矮缩病毒S6编码一个沉默抑制子被引量：25: 《中国农业科学》2011年第14期2909-2917,共9页卢嫣红张金凤熊如意徐秋芳周益军; 国家973计划(2010CB126203);国家自然科学基金(31000841);国家转基因生物新品种培育重大专项(2009ZX08001-013B,2009ZX08001-019B);国家公益性行业专项(201003031);江苏省自然科学基金(BK2010018); 【目的】分析南方水稻黑条矮缩病毒(SRBSDV)基因组S6编码的SP6蛋白的抑制子活性,明确SRBSDV是否编码RNA沉默抑制子来干扰植物的沉默。【方法】将分别含有SP6与GFP质粒的农杆菌共浸润转GFP基因的16c本氏烟纯合系,观察SP6对局部沉默和系...; 关键词：南方水稻黑条矮缩病毒 SP6 基因沉默抑制子

Transmission of Rice Black-Streaked Dwarf Virus from Frozen Infected Leaves to Healthy Rice Plants by Small Brown Planthopper (Laodelphax striatellus)被引量：3: 《Rice science》2011年第2期152-156,共5页ZHou Tong Wu Li-juan WANG Ying CHENG Zhao-bang JI Ying-hua FAN Yong-jian Zuou Yi-jun; supported by the National Basic Research Program(Grant No.2010CB126203);Special Fund for Agro-scientific Research in the Public Interest(Grant No. 201003031);the National Natural Science Foundation of Jiangsu Province,China(Grant Nos.BK2009325 and BK2010018); In order to preserve virus for identifying the resistance of rice varieties against rice black-streaked dwarf disease, a simple and reliable method was developed, through which virus-free small brown planthopper （SBP...; 关键词：rice black-streaked dwarf virus frozen infected leaves small brown planthopper methodology

Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus被引量：7: 《Science China(Life Sciences)》2011年第3期201-208,共8页WU JianGuo WANG ChunZheng DU ZhengGuo CAI LiJun HU MeiQun WU ZuJian LI Yi XIE LianHui; supported by the National Basic Research Program of China(Grant No. 2010CB126203);the National Transgenic Major Program(Grant Nos. 2009ZX08009-044B and 2009ZX08001-018B);the National Natural Science Foundation of China(Grant No. 30970135);the Fujian Province Education Department(Grant No. JB08078);Specialized Research Fund for the Ministry of Agriculture(Grant No. nyhyzx07-051); RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms.It has been used to regulate gene expression and development.In addition,RNA silencing serves as an important mechanism in plants' defe...; 关键词：Rice gall dwarf virus Pns12 silencing suppressor

水稻瘤矮病毒S12编码第2个RNA沉默抑制子被引量：1: 《中国科学：生命科学》2011年第1期61-69,共9页吴建国王春政杜振国蔡丽君胡梅群吴祖建李毅谢联辉; 国家重点基础研究发展计划(批准号:2010CB126203);国家科技重大专项(批准号:2009ZX08009-044B;2009ZX08001-018B);国家自然科学基金(批准号:30970135);福建省教育厅项目(批准号:JB08078);农业部农业公益性行业科研专项基金(批准号:nyhyzx07-051)资助; RNA沉默是一种保守而普遍存在于真核生物体内用于调控基因表达和发育的一种机制,也是植物抵抗病毒侵染和转录的一种防御机制.作为一种反防御机制,许多植物病毒编码沉默抑制子.本研究利用农杆菌共浸润的方法发现,RGDV基因组S12片段编码的...; 关键词：水稻瘤矮病毒(RGDV) Pns12 RNA沉默抑制子

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