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国家自然科学基金(30500224): 作品数：11被引量：23H指数：2; 导出分析报告; 相关作者：张惠兰张珍祥徐永健郦俊熊盛道更多>>; 相关机构：华中科技大学更多>>; 相关期刊：《华中科技大学学报（医学版）》《Chinese Medical Journal》《中国病理生理杂志》更多>>; 相关主题：ALVEOLARVASCULAR_ENDOTHELIAL_GROWTH_FACTORA549EPITHELIALASTHMA更多>>; 相关领域：医药卫生更多>>

Establishment of Surfactant-associated Protein A Suicide Gene System and Analysis of Its Activity: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2014年第3期337-342,共6页张万广何丽苏化庆史学梅张波吴思思梅丽 Katirai Foad 徐永健张珍祥赵建平熊维宁甄国华张惠兰; supported by the National Natural Science Foundation of China(No.30500224); Summary： Alveolar epithelial type II （AT II） cells are essential for lung development and remodeling, as they are precursors for type I cells and also produce other non-repair cells （fibroblasts）. Progenitor ceil...; 关键词：pulmonary surfactant-associated protein A alveolar epithelial type II cells suicide gene system NICHE

Murine calcium-activated chloride channel family member 3 induces asthmatic airway inflammation independently of allergen exposure: 《Chinese Medical Journal》2013年第17期3283-3288,共6页MEI Li HE Li WU Si-si ZHANG Bo XUYong-jian ZHANG Zhen-xiang ZHAO Jian-ping ZHANG Hui-lan; This work was supported by a grant from the National Natural Science Foundation of China （No. 30500224）.; Background Expression of murine calcium-activated chloride channel family member 3 （mCLCA3） has been reported to be increased in the airway epithelium of asthmatic mice challenged with ovalbumin （OVA）. However, it...; 关键词：murine calcium-activated chloride channel family member 3 ASTHMA INFLAMMATION

SPA基因启动子的克隆及转录靶向活性分析被引量：2: 《中国病理生理杂志》2008年第10期2002-2007,共6页史雪梅张惠兰熊盛道甄国华熊维宁张珍祥徐永健胡琼洁赵建平倪望; 国家自然科学基金资助项目(No.30400193);国家自然科学基金资助项目(No.30500224); 目的:克隆大鼠肺表面活性蛋白A(SPA)基因启动子并构建该启动子的荧光报告系统,探讨该启动子的活性及转录靶向性,为进一步研究SPA基因的表达调控机制和探讨靶向性基因治疗奠定基础。方法:①从GenBank中获取大鼠SPA基因序列,对其上游基因...; 关键词：基因 SPA 启动子基因表达转录靶向性肺表面活性蛋白

Altered surfactant protein A gene expression and protein homeostasis in rats with emphysematous changes: 《Chinese Medical Journal》2008年第13期1177-1182,共6页HU Qiong-jie XIONG Sheng-dao ZHANG Hui-lan SHI Xue-mei XU Yong-jian ZHANG Zhen-xiang ZHEN Guo-hua ZHAO Jian-ping; This work was supported by grants from the National Natural Science Foundation of China （No. 30500224, 3040199）.; Background The decrease of surfactant protein （SP） secreted by the alveolar type Ⅱ cell is one of the important causes of limiting air of pulmonary emphysema. However, the SP-A gene and protein changes in this dise...; 关键词：EMPHYSEMA alveolar type Ⅱ cells surfactant protein A gene HOMEOSTASIS

The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke被引量：2: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2008年第2期128-131,共4页胡琼洁张惠兰熊盛道史雪梅徐永健张珍祥甄国华赵建平; grants from the National Natural Sciences Foundation of China (No. 30500224, 3040199); In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural...; 关键词：SMOKING alveolar type Ⅱ cells SP-A IMMUNOHISTOCHEMISTRY mRNA

Primary Culture of Alveolar Epithelial Type Ⅱ Cells and Its Bionomic Study被引量：1: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2007年第6期653-656,共4页史雪梅张惠兰熊盛道甄国华熊维宁张珍祥徐永健胡琼洁赵建平倪望; the National Natural Sciences Foundation of China (No. 30500224 and No. 30400193).; To establish a better method of primary culture for alveolar epithelial type Ⅱ cells （AEC Ⅱ ） and to study its bionomics, alveolar epithelial type Ⅱ cells were isolated by digestion with trypsin and collagenase, ...; 关键词：alveolar epithelial type Ⅱ cells primary culture BIONOMICS

The Effect of Interleukin-18 on Airway Inflammation in Asthmatic Murine Models and Its Mechanisms: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2007年第5期501-504,共4页张惠兰张珍祥徐永健邢丽华刘剑波; The project was supported by a grant from the National Natural Sciences Foundation of China (No 30500224); In order to investigate the effect of interleukin-18 （IL-18） on airway inflammation in asthmatic murine models and its mechanisms, BALB/C mice were randomly divided into three groups （n=10 in each group）： group A...; 关键词：interleukin- 18 ASTHMA EOSINOPHIL plasmacyte interferon gamma

缺氧诱导因子-1α增加肿瘤细胞A549化疗药物耐受性的研究被引量：2: 《华中科技大学学报（医学版）》2007年第5期578-580,584,共4页张惠兰郦俊张珍祥徐永健; 国家自然科学基金资助项目(No30500224); 目的构建缺氧诱导因子-1α(hypoxia-inducible factor-1alpha,HIF-1α)基因的表达质粒,转染肺癌细胞系A549后,观察其对化疗药物5-氟尿嘧啶(5-Fu)敏感性的变化。方法采用RT-PCR方法扩增A549细胞内HIF-1α基因功能区片段,克隆至真核表达载...; 关键词：缺氧诱导因子1Α 化疗肺癌

Antisense Oligonucleotide of Hypoxia-inducible Factor-1alpha Suppresses Growth and Tumorigenicity of Lung Cancer Cells A549被引量：2: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第4期448-450,共3页张万广张惠兰邢丽华; This project was supported by a grant from the National Natural Science Foundation of China (No. 30500224); Summary： To study the role and mechanisms of hypoxia-inducible factor-lalpha （HIF-1α on the growth and tumorigenicity of lung cancer cells A549, the antisense oligonucleotide of HIF-1α was transfected to A549 cell...; 关键词：hypoxia inducible factor-lalpha antisense oligonucleotide vascular endothelial growth factor

Expression and Significance of Bcl-2,Bax,Fas and Caspase-3 in Different Phases of Human Hemangioma被引量：13: 《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第4期402-404,共3页杨虹邓成国陕声国张端莲余瑛; This project was supported by a grant from the NationalNatural Science Foundation of China (No. 30500224); Summary： The relationship between Bcl-2, Bax, Fas, caspase-3 and development of hemangioma and the molecular mechanism was investigated. By using immunohistochemical S-P method, proliferating cell nuclear antigen was...; 关键词：hypoxia inducible factor-lalpha antisense oligonucleotide vascular endothelial growth factor

国家自然科学基金(30500224)