上海市教育委员会重点学科基金(B111)

作品数:27被引量:59H指数:5
导出分析报告
相关作者:钟扬黄强南蓬韩利平王庆彪更多>>
相关机构:复旦大学上海生物信息技术研究中心西藏大学中国科学院更多>>
相关期刊:《Genomics, Proteomics & Bioinformatics》《科学通报》《生物物理学报》《自然科学进展》更多>>
相关主题:PHYLOGENY青蒿素RBCL基因适应性进化DNA序列更多>>
相关领域:生物学医药卫生理学农业科学更多>>
-

检索结果分析

结果分析中...
条 记 录,以下是1-10
视图:
排序:
一种碟式微流控结晶芯片与传统结晶板的蛋白质结晶能力比较
《复旦学报(自然科学版)》2015年第6期802-808,共7页孙凯雷 王丽 杨文静 祁祺 胡小健 李刚 
国家重点基础研究发展计划(2012CB933303;2011CB707505);上海市科学技术委员会(13DZ2252000);上海市重点学科建设(B111);国家自然科学基金(61271161;61271162);国家基础科学人才培养基金(J1210012)
设计并制作了一种在微腔中对蛋白质进行微批量法(microbatch)结晶的碟式微流控结晶芯片,它是一种高通量、低成本、低耗样量(纳升级)、操作简便的蛋白质结晶筛选方法,这种芯片能成功地结晶溶菌酶(lysozyme)和青色荧光蛋白(CyPet).为了系...
关键词:微流控结晶芯片 悬滴蒸汽扩散 蛋白质结晶 
The in vitro interaction of CmeA with CmeC
《Chinese Science Bulletin》2014年第25期3114-3119,共6页Hua-Wei Zhang Ximin Zeng Qi Qi Kai-Lei Sun Chong-Jun Ma Xiao-Jian Hu Jun Lin 
supported by the National Basic Research Program of China(2009CB918602);the National Natural Science Foundation of China(30970575 and 31011120381);Shanghai Leading Academic Discipline Project(B111);Qi Qiwas supported by the National Talent Training Fund in Basic Research of China(J1210012)
The membrane fusion protein CmeA and the outer membrane channel CmeC,two important components of CmeABC system in Campylobacter jejuni,were expressed in Escherichia coli.After Ni-NTA and ion exchange columns purificat...
关键词:相互作用 体外 等温滴定量热法 尺寸排阻色谱 大肠杆菌 膜融合蛋白 空肠弯曲菌 ABC系统 
柔性多肽片段–蛋白质相互作用的全局对接方法被引量:1
《计算生物学》2014年第2期42-52,共11页来瑞颖 万波 黄强 
上海市重点学科建设项目(B111)。
多肽–蛋白质的相互作用在生物细胞中发挥着各种各样重要的作用。通常情况下,它们之间的结合信息是未知的。所以,利用计算方法预测结合位点具有重要意义。而以Rosetta为代表的常用对接软件通常具有很强的初始位置依赖性。为克服这一局限...
关键词:多肽–蛋白质相互作用 多肽结合位点 分子对接 
DNA聚合酶与引物/模板的相互作用对PCR效率的影响(英文)被引量:1
《中国生物工程杂志》2014年第5期6-13,共8页杨奇奇 张俊威 朱坚 刘建平 黄强 
Supported by the grants from the Shanghai Leading Academic Discipline Project(B111);the Shanghai Natural Science Foundation(13ZR1402400)~~
PCR是体外酶促合成特异DNA片段的一种方法,引物的优劣直接关系到PCR的特异性与成功与否。传统的PCR引物设计软件基本上忽略了DNA聚合酶与引物/模板的亲和性对PCR效率的影响。为揭示DNA聚合酶与引物/模板的相互作用是否对PCR的效率有影响...
关键词:PCR DNA聚合酶 引物设计 结合自由能 分子建模 
Shigella Strains Are Not Clones of Escherichia coli but Sister Species in the Genus Escherichia被引量:6
《Genomics, Proteomics & Bioinformatics》2013年第1期61-65,共5页Guanghong Zuo Zhao Xu Bailin Hao 
supported by the National Basic Research Program of China (973 Project, Grant No. 2007CB814800 and2013CB834100);the Shanghai Leading Academic Discipline Project (Grant No. B111);the National Key Laboratory of Applied Surface Physics and the Department of Physics, Fu-dan University
Shigella species and Escherichia coli are closely related organisms. Early phenotyping experiments and several recent molecular studies put Shigella within the species E. coli. However, the whole-genome-based, alignme...
关键词:SHIGELLA Escherichia coli Prokaryote phylogeny andtaxonomy Composition vector CVTree 
Asymmetric transportation induced by thermal noise at the nanoscale被引量:3
《Science China(Physics,Mechanics & Astronomy)》2012年第5期751-756,共6页WAN RongZheng HU Jun FANG HaiPing 
supported by the National Natural Science Foundation of China (Grant Nos.10825520 and 11175230);the Shanghai Leading Academic Discipline Project (B111);the Knowledge Innovation Program of the Chinese Academy of Sciences;the Shanghai Supercomputer Center of China
Based on a simple model, we theoretically show that asymmetric transportation is possible in nanoscale systems experiencing thermal noise without the presence of extemal fluctuations. The key to this theoretical advan...
关键词:RATCHET NANOSCALE thermal fluctuations 
Calculating pH-dependent free energy of proteins by using Monte Carlo protonation probabilities of ionizable residues被引量:1
《Protein & Cell》2012年第3期230-238,共9页Qiang Huang Andreas Herrmann 
This study was supported in part by grants from the National Natural Sci-ence Foundation of China(Grant No.30570406);the HI-tech Re-search and Development Program of China(Grant No.2008AA02Z311);the Shanghai Leading Academic Discipline Project(B111).
Protein folding,stability,and function are usually in-fluenced by pH.And free energy plays a fundamental role in analysis of such pH-dependent properties.Elec-trostatics-based theoretical framework using dielectric so...
关键词:protein protonation protein electrostatics pH-dependent free energy Poisson-Boltzmann equation Monte Carlo simulation 
Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro被引量:2
《Protein & Cell》2012年第2期153-159,共7页Juan Li Bin Liu Xiaofei Gao Zhixing Ma Tianyi CaoSong Yan-ai Mei Yufang Zheng 
by the Shanghai Leading Academic Discipline Project(B111);NSFC(Grant No.30800322);Shanghai Pujiang Program(08PJ1401300);Ministry of Education Start Fund to Returned Overseas Scholars;Ministry of Education Research Fund for New Teachers in Doctoral Program of Higher Educational Institutes;National Basic Research Program of China(973 Program)(Grant No.2011CB503703);and Institute of Brain Sciences at Fudan University.The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
The sigma-1 receptor is a molecular chaperone protein highly enriched in the brain.Recent studies linked it to many diseases,such as drug addition,Alzheimer’s disease,stroke,depression,and even cancer.Sigma-1 recepto...
关键词:sigma-1 receptor ADAM17 ADAM10 SHEDDING lipid raft 
人染色体8p11(CHRNB3-CHRNA6)区域基因多态性与中国汉族人群肺癌易感性的相关性被引量:3
《遗传》2011年第8期886-894,共9页张晓博 赵振宏 陈红岩 王久存 钱吉 杨亚军 魏庆义 黄建 卢大儒 
上海市科委重点项目(编号:09JC1402200);上海市国际合作项目(编号:10410709100);上海市重点学科建设项目(编号:B111)资助
为探讨人染色体8p11(CHRNB3-CHRNA6)区域基因多态性与中国汉族人群肺癌遗传易感性之间的关系,文章采用病例-对照研究,对784例肺癌患者和782例性别、年龄、籍贯频数与之相匹配的健康对照中该区域6个标签SNP位点进行基因分型,并统计分...
关键词:8p11 CHRNB3 CHRNA6 单核苷酸多态性 肺癌易感性 
建立标准化SEAP报告基因系统的研究
《西北师范大学学报(自然科学版)》2011年第3期75-79,115,共6页朱青 卢大儒 
上海市重点学科建设项目(B111);上海市国际合作项目(10410209100);上海市科委重点项目(09JC1402200)
通过建立SEAP(分泌性碱性磷酸酶)产物的标准曲线,得出SEAP的吸光值与酶活力、蛋白质绝对值之间相互换算的公式,从酶动力常数、酶反应时间、不同量mRNA与酶吸光值之间的对应关系等方面进行研究.建立了在405 nm波长处,SEAP的吸光值与酶活...
关键词:SEAP 报告基因 酶活力标准 
检索报告 对象比较 聚类工具 使用帮助 返回顶部