WHOLE-CELL

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Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst被引量：1
《Synthetic and Systems Biotechnology》2023年第4期741-748,共8页Chixiang Sun Baodong Hu Yanchun Li Zhimeng Wu Jingwen Zhou Jianghua Li Jian Chen Guocheng Du Xinrui Zhao 
supported by the National Key Research and Development Program of China(2019YFA0906400);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-08);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX23_2486);We thank Prof.Shengying Li(Shandong University,China)for providing plasmids pET28a-SelFdx1499 and pET28a-SelFdR0978.
Deoxycholic acid(DCA)has been authorized by the Federal Drug Agency for cosmetic reduction of redundant submental fat.The hydroxylated product(6β-OH DCA)was developed to improve the solubility and pharmaceutic proper...
关键词：OleP Deoxycholic acid HYDROXYLATION Redox partners Whole-cell catalysis 
Enantioselective Resolution of (±)-l-Phenylethyl Acetate by Using the Whole Cells of Deep-sea Bacterium Bacillus sp. DL-2被引量：2
《Chemical Research in Chinese Universities》2019年第5期792-798,共7页DONG Lu XU Yongkai ZHANG Yun SUN Aijun HU Yunfeng 
Supported by the Natural Science Foundation of Guangdong Province,China(No.2018A030313151);the Senior User Project of the Research Vessel KEXUE(No.KEXUE2018G05);the Priority Research Program of the Chinese Academy of Sciences(No.XDAl 1030404);the Scientific and Technological Project of the Ocean and Fishery from Guangdong Province,China(No.A201701C12).
Bacillus sp. DL-2 was isolated from the deep sea of the Western Pacific and further utilized as novel biocatalysts to efficiently asymmetrically hydrolyze (±)-1-phenylethyl acetate. After the optimization of hydrolyt...
关键词：DEEP-SEA MICROORGANISM WHOLE-CELL BIOCATALYST Asymmetric hydrolysis (R)-1-Phenylethanol (S)-1-Phenylethyl acetate 
Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity被引量：1
《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2011年第7期545-551,共7页Mei-ling CHEN Qin GUO Rui-zhi WANG Juan XU Chen-wei ZHOU Hui RUAN Guo-qing HE 
Project supported by the National High-Tech R & D Program (863) of China (No. 2006AA10Z308);the National Science Foundation of China (No. 20776130);the Zhejiang Provincial Natural Science Foundation of China (No. Y4090309);the Zhejiang Provincial Science and Technology Program of China (No. 2009C32009)
Surface display is effectively utilized to construct a whole-cell biocatalyst.Codon optimization has been proven to be effective in maximizing production of heterologous proteins in yeast.Here,the cDNA sequence of Rhi...
关键词：Rhizopus oryzae lipase （ROL） Yeast surface display Codon optimization Whole-cell biocatalyst 
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