CULTURE-INDEPENDENT

作品数：11被引量：17H指数：3
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Recent advances in the culture-independent discovery of natural products using metagenomic approaches
《Chinese Journal of Natural Medicines》2024年第2期100-111,共12页SHEN Yiping LIU Nan WANG Zongqiang 
supported by the Start-Up Grant from China Pharmaceutical University(No.3154070046).
Natural products derived from bacterial sources have long been pivotal in the discovery of drug leads.However,the cultivation of only about 1%of bacteria in laboratory settings has left a significant portion of biosyn...
关键词：METAGENOMICS Culture-independent approach Natural product Functional screening Sequence tag-based homology screening Syn-BNP 
Development and performance evaluation of a culture-independent nanopore amplicon-based sequencing method for accurate typing and antimicrobial resistance profiling in Neisseria gonorrhoeae
《Science China(Life Sciences)》2024年第2期421-423,共3页Chi Zhang Lulu Zhang Feng Wang Yaling Zeng Liying Sun Di Wang Yamei Li Liqin Wang Junping Peng 
supported by the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-I2M-1-037,2021-I2M-1-038);the National Natural Science Foundation of China(82002193);the National Science and Technology Infrastructure of China(National Pathogen Resource Center-NPRC-32);the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019-PT310-029,2021-PT310-004);the Fundamental Research Funds for the Central Universities(3332021092)。
Dear Editor,Bacterial antimicrobial resistance(AMR)poses a serious threat to global human health(Antimicrobial Resistance Collaborators,2022).Comprehensive profiling of AMR and accurate molecular typing are important ...
关键词：GONORRHOEAE resistance 
Culture-independent assessment of probiotic supplement consistency in commercially available probiotic supplements
《Food Bioscience》2023年第3期2118-2125,共8页Aycan Gundogdu O.Ufuk Nalbantoglu Ilknur Sarikaya Gizem Karis Meryem N.Erdogan Mehmet Hora Varol Tunali 
The probiotic products market has experienced significant growth in recent years,driven by increasing consumer demand for health benefits.This demand is largely fueled by the widespread promotion of probiotic products...
关键词：Probiotic products Shotgun metagenome sequencing Antibiotic-resistance genes Label discrepancies 
Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches被引量：5
《Fungal Diversity》2018年第3期1-84,共84页Ruvishika S.Jayawardena Witoon Purahong Wei Zhang Tesfaye Wubet XingHong Li Mei Liu Wensheng Zhao Kevin D.Hyde JianHua Liu Jiye Yan 
s This work was financially supported by Beijing Talent Programm for Jiye Yan,CARS-29 and JNKYT201605.
This study is unique as it compares traditional and high-resolution culture-independent approaches using the same set of samples to study the saprotrophic fungi on Vitis vinifera.We identified the saprotrophic communi...
关键词：CHECKLIST GRAPEVINE Mycobiome Next generation sequencing PATHOGENS Saprotrophs 
Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine(Vitis vinifera)被引量：4
《Fungal Diversity》2018年第3期85-107,共23页Asha J.Dissanayake Witoon Purahong Tesfaye Wubet Kevin D.Hyde Wei Zhang Haiying Xu Guojun Zhang Chunyuan Fu Mei Liu Qikai Xing Xinghong Li Jiye Yan 
This work was financially supported by Beijing Talent Program for Dr.Jiye Yan,CARS-29,Beijing science and technology project D17110001617002;We thank Dr.Heng Gui for his support to submit Raw Illumina reads to the Sequence Read Archive(SRA)of National Center for Biotechnology Information(NCBI).
Grapevines(Vitis vinifera)are colonized by ubiquitous microorganisms known as endophytes,which may have advantageous or neutral effects without causing disease symptoms.Certain endophytes are uncultivable,so culture-...
关键词：Molecular data Morphological characteristics Multi-gene phylogeny Mycobiome Next generation sequencing 
A Study of the Microbial Community at the Interface between Granite Bedrock and Soil Using a Culture-Independent and Culture-Dependent Approach被引量：1
《Advances in Microbiology》2016年第3期233-245,共13页Karen Olsson-Francis Victoria K. Pearson Paul F. Schofield Anna Oliver Stephen Summers 
The dissolution of minerals plays an important role in the formation of soils and sediments. In nutrient limiting soils, minerals constitute a major reservoir of bio-essential cations. Of particular interest is granit...
关键词：Mineral Weathering Soil Community 
A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface被引量：2
《Advances in Microbiology》2015年第13期842-857,共16页Karen Olsson-Francis Carl P. Boardman Victoria K. Pearson Paul F. Schofield Anna Oliver Stephen Summers 
In nutrient limited soils, minerals constitute a major reservoir of bio-essential elements. Consequently, the release of nutritive elements during weathering is crucial. Bacteria have been shown to enhance weathering ...
关键词：Mineral WEATHERING Soil Microbial COMMUNITIES BASALT DISSOLUTION 
Marine Metagenome as A Resource for Novel Enzymes被引量：2
《Genomics, Proteomics & Bioinformatics》2015年第5期290-295,共6页Amani D.Alma'abadi Takashi Gojobori Katsuhiko Mineta 
supported by King Abdullah University of Science and Technology (KAUST),Saudi Arabia
More than 99% of identified prokaryotes, including many from the marine environment, cannot be cultured in the laboratory. This lack of capability restricts our knowledge of microbial genetics and community ecology. M...
关键词：Microbial diversity Culture-independent studies CATALYSIS LIPASE BIOTECHNOLOGY 
Bacterial diversity in Arctic marine sediment determined by culture-dependent and-independent approaches
《Advances in Polar Science》2014年第1期46-53,共8页LIN Xuezheng WANG Zhen CHE Shuai SONG Weizhi YI Dan 
financially supported by the Public Science and Technology Funds for Ocean Projects(Grant nos.201005032-2 and 201205020-5);by the Special Funds for Basic Scientific Research by Central Non-profit Institutes(Grant no.GY02-2012G14)
Bacterial diversity in surface sediment from the Arctic Ocean was investigated by culture-dependent and-independent approaches. Conventional culture-dependent techniques revealed 11 strains based on their distinct mor...
关键词：Arctic marine sediment bacterial diversity culture-dependent CULTURE-INDEPENDENT 
Fungal diversity in adult date palm(Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches
《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第12期1084-1099,共16页Ines BEN CHOBBA Amine ELLEUCH Imen AYADI Lamia KHANNOUS Ahmed NAMSI Frederique CERQUEIRA Noureddine DRIRA Néji GHARSALLAH Tatiana VALLAEYS 
supported by EGIDE(No.18470SA),CMCU(No.08G908);the Tunisian Ministry of Higher Education
Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various ...
关键词：Date palm tree(Phoenix dactylifera) Endophytic cultivable fungi rDNA internal transcribed spacer(ITS) Phylogenetic analysis Total DNA diversity analysis 
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