COFACTOR

作品数:50被引量:72H指数:4
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Peptide bridging for cofactor channeling in fusion enzyme lowers cofactor input by two orders of magnitude
《Chinese Journal of Catalysis》2025年第4期390-403,共14页Zehui Guo Yan Sun 
国家重点研发计划(2018YFA0900702).
Biocatalysis with nicotinamide adenine dinucleotide phosphate(NADP)-dependent oxidoreductases faces a challenge in improving the efficiency of the costly cofactor utilization.Although enzyme fusion can offer cofactor ...
关键词:Peptide bridge Fusion enzyme NADP(H)-dependent oxidoreductases Cofactor channeling Cofactor regeneration 
Enhanced cofactor recycling and accelerated reaction rate via liquid-liquid phase separation in dual-enzyme condensates
《Chinese Journal of Catalysis》2025年第2期135-148,共14页Jiaxu Liu Jiaxin Chen Xiaoyan Zhang Daidi Fan Yunpeng Bai 
国家重点研发计划(2023YFA0913600);国家自然科学基金(22378120,22078096)。
Enzyme catalysis is a promising way to produce chiral products in a green and sustainable way.However,the high cost of cofactors and their relatively low recycling efficiency hinder the widespread application of enzym...
关键词:Cofactor recycling CONDENSATE Proximity effect Intrinsically disordered region Enzyme catalysis 
Double-stranded DNA-nucleic acid dye complex-sensitized biocatalytic photosynthesis:Base pairing-switched regeneration of nicotinamide cofactor
《Journal of Energy Chemistry》2024年第7期618-624,共7页Zhen Dong Yanying Wang Dan Li Peng Wu 
the financial support from the National Natural Science Foundation of China (22274102 and 22325403);the Fundamental Research Funds from Sichuan University (2022SCUNL104);the Open Research Fund of School of Chemistry and Chemical Engineering,Henan Normal University(2022A02)
Photo-biocatalysis,the combination of photosensitization and biocatalysis,is an emerging solution for sunlight-based renewable energy.It is thus important to develop light antennas with both good light har-vesting and...
关键词:PHOTOSYNTHESIS DSDNA SYBRGreenI TICT 2-phenylpropanol 
Engineering of cofactor preference and catalytic activity of methanol dehydrogenase by growth-coupled directed evolution被引量:2
《Green Carbon》2024年第2期242-251,共10页Jinxing Yang Liwen Fan Guimin Cheng Tao Cai Jibin Sun Ping Zheng Shuang Li Yu Wang 
supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDC0110201);the National Key R&D Program of China(2018YFA0901500);the National Natural Science Foundation of China(32070083 and 32222004);the Innovation Fund of Haihe Laboratory of Synthetic Biology(22HHSWSS00017);the Youth Innovation Promotion Association of Chinese Academy of Sciences(2021177);the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-008).
Methanol,produced from carbon dioxide,natural gas,and biomass,has drawn increasing attention as a promising green carbon feedstock for biomanufacturing due to its sustainable and energy-rich properties.Nicotinamide ad...
关键词:Growth-coupled screening Methanol dehydrogenase Cofactor engineering Directed evolution C1 bioconversion 
Copper-radical oxidases: A diverse group of biocatalysts with distinct properties and a broad range of biotechnological applications
《Engineering Microbiology》2022年第3期23-36,共14页Katja Koschorreck Saadet Alpdagtas Vlada B.Urlacher 
This work was supported by the Ministry of Innovation,Science and Research the state of North Rhine-Westphalia,Germany within the framework of the NRW-Strategieprojekt BioSC(No.313/323-400-00213);together with the“European Regional Development Fund(EFRE)”,Project“Clus-ter Industrial Biotechnology(CLIB)Kompetenzzentrum Biotechnologie(CKB)”(34.EFRE-0300095/1703FI04);Financial support by the Scien-tific and Technological Research Council of Turkey(TUBITAK)2219-International Research Fellowship Programme for Saadet Alpdagtas is gratefully acknowledged.
Copper-radical oxidases(CROs)catalyze the two-electron oxidation of a large number of primary alcohols includ-ing carbohydrates,polyols and benzylic alcohols as well as aldehydes and𝛼-hydroxy-carbonyl compounds while...
关键词:Copper-radical oxidases Galactose oxidase Glyoxal oxidase Cysteine-tyrosyl radical cofactor Carbohydrates Alcohols Aldehydes 
Improved L -phenylglycine synthesis by introducing an engineered cofactor self-sufficient system
《Synthetic and Systems Biotechnology》2022年第1期513-521,共9页Pengchao Wang Xiwen Zhang Yucheng Tao Xubing Lv Shengjie Cheng Chengwei Liu 
the National Natural Science Foundation of China(Project No.31900064);as well as the Natural Science Foundation of Heilongjiang Province of China(Project No.LH2019C012)。
L-phenylglycine(L-phg)is a valuable non-proteinogenic amino acid used as a precursor to β-lactam antibiotics,antitumor agent taxol and many other pharmaceuticals.L-phg synthesis through microbial bioconversion allows...
关键词:L-phenylglycine Whole-cell biocatalyst Self-sufficient Protein scaffold Hydroxymandelate synthase 
Conformational conversion of prion protein in prion diseases被引量:3
《Acta Biochimica et Biophysica Sinica》2013年第6期465-476,共12页Zheng Zhou Gengfu Xiao 
Funding This work was supported by the grants from the National Natural Science Foundation of China (No. 30970150 and No. 31170156).
Prion diseases are a group of infectious fatal neurodegenerative diseases. The conformational conversion of a cellular prion protein (PrPc) into an abnormal misfolded isoform (PrPso) is the key event in prion dise...
关键词:prion disease prion protein conformationalconversion COFACTOR 
DFT Study on One-carbon Unit Transfer from 1,10-CH^+- tetrahydroquinoxaline to Methylamine
《Chinese Chemical Letters》2003年第1期72-75,共4页Chuan Song QI Da Cheng FENG Hua Yang WANG Zheng Ting CAI 
This work was supported by the grant from the National Natural Science Foundation of China (No. 29973021)
Density Functional Theory (DFT) method was used in this paper to study one-carbon transfer from 1,10-tetrahydroquinoxaline, an analogue of tetrahydrofolic acid, to methylamine. This reaction can be completed via two ...
关键词:Density Functional Theory (DFT) folate cofactor tetrahydroquinoxaline one-carbon unit transfer. 
Quantum chemical study on the one-carbon unit transfer of imidazolinium
《Science China Chemistry》2002年第3期257-266,共10页康从民 冯大诚 戚传松 蔡政亭 
This work was supported by the National Natural Science Foundation of China (Grant No. 29973021).
One-carbon unit transfer reaction of folate cofactor model compound, 1-acetyl-2-methyl-imidazolinium, with 1,2-diaminobenzene has been studied theoretically with ONIOM method. The result shows that there are two pathw...
关键词:folate COFACTOR model one-carbon UNIT TRANSFER imidazolinium ONIOM method. 
Studies on Chem icalCom position ofIron-m olybdenum Cofactor fromNitrogenase by Fluorescence Analysis
《Chemical Research in Chinese Universities》1999年第3期286-293,共8页ZHANG Feng-zhang XU Liang-shu HUANG He-qing LONG Min-nan QIU Xue-hui CHEN Zhong 
The iron molybdenum cofactor(FeMoco) of nitrogenase MoFe protein from Azotobacter vinelandii OP was extracted by N methylformamide(NMF). The effects of FeMoco(in NMF) on electronic spectrum and fluorescence...
关键词:NITROGENASE Iron molybdenum cofactor Fluorophotometric titration Structural change 
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