The surface of silica gels was modified by an diethylamino group and an hydroxy group, simultaneously. The activity of the aminoacylase immobilized on such amino-hydroxy-silica gels was 316.8 U/g. By its catalysis, th...
The kinetic method of the substrate reaction in the presence of mactivator previously described by Tsou has been applied to the determination of inactivation rates of aminoacylase during denaturation in urea solutions...
Aminoacylase is a dimeric metal enzyme containing one Zn^(2+)-ion per subunit of active site. It is essential for the activity of enzyme.Fourier transform-infrared spectroscopy has been used for the study on the secon...
<正> Synthesized macroporous cross-linked copolymers of methyl acrylate-divinyl benzene (MA-DVB), acrylamide-N,N’-methylenebisacrylamide (AAM-BIS) and their functionalized products were used for immobilization of ami...
Macroporous methyl acrylate-divinyl benzene copolymers were synthesized and modified by polyethylene polyamine.The aminoacylase from Aspergillus oryzae was adsorded on these modified copolymers.The role of certain fac...
The kinetics theory of the substrate reaction during modification of enzyme activity previously described by Tsou has been applied to a study on the kinetics of the course of inactivation of aminoacylase I by DPDS and...
A number of chlorumethylated polystyrenes were synthesized and tried to immobilize aminoacylase from Aspergillus oryzae and many factors which affected immobilized enzyme activity were studied in detail. The results ...
<正> A polymer with suitable physical characters and matched functional groups was synthesized,and used as a supporter to immobilize aminoacylase. The results showed that this supporterhad high immobilizing capacity a...