the Ministry of Science and Technology of China (2019YFA0508400 and 2016YFA0500700);the National Natural Science Foundation of China (21874123 and 32071220);the Collaborative Innovation Program of Hefei Science Center, CAS (2020HSC-CIP009), for financial support
The assembly of biomolecular condensates is driven by liquid–liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g...
We reconstituted bilayer nuclear membranes, multilayer membranes, and organelles from mixtures ofXenopus laevis egg extracts and demembranatedXenopus sperm nuclei. Varying proportions of the cytosolic and vesicular fr...