PICHIA_PASTORIS

作品数：195被引量：497H指数：10
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  • 期刊=Chemical Research in Chinese Universitiesx
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Expression and Activities Experiment of DNA Transduction Motif Based on GAL4 in Pichia Pastoris
《Chemical Research in Chinese Universities》2010年第2期221-224,共4页XU Xiao-hong CHI Bao-rong LI Xiao YANG En-cheng GAO Peng LIU Yan JIA Peng KAN Shi-fu WEN Zong-mei JIN Ning-yi 
Supported by National High-tech Research and Development Program of China(No.2007AA021004)
The genes encoding DNA-binding domain（BD） designed based on the yeast transcriptional activator GAL4 and protein transduction domain of HIV-1 Tat protein were fused via soft linker peptide sequence, and cloned into ...
关键词：Nonviral DNA delivery Yeast transcription activator（GAL4） Cell-penetrating peptide Upstream activating sequence（UAS） Secrete expression Pichia pastoris 
Expression and Characterization of a Recombinant Truncated Capsid Protein of Hepatitis E Virus in Pichia pastoris被引量：2
《Chemical Research in Chinese Universities》2010年第2期235-239,共5页YANG En-cheng CHI Bao-rong LI Xiao LIU Yan GAO Peng JIA Peng KAN Shi-fu WEN Zhong-mei WANG Wan JIN Ning-yi 
Supported by the National Natural Science Foundation of China(No.30771609);the National High-tech Research and Development Program of China(No.2007AA021004)
Hepatitis E is an enterically transmitted viral disease caused by infection with hepatitis E virus（HEV）. HEV is a nonenveloped virus that bas been classified in the family of Caliciviridae. The virus appears to be a...
关键词：Hepatitis E virus Capsid protein PICHIAPASTORIS Protein purification 
Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichia pastoris被引量：2
《Chemical Research in Chinese Universities》2010年第2期240-244,共5页QU He-zhi DU Shan-shan YANG Shuo HUANG Lu ZHANG Lei XIAO Song HAO Dong-yun WANG Xiao-ping 
Mammalian cells express two isoforms of Cu- and Zn-containing superoxide dismutases（SODs）, CuZn-SOD and extracellular SOD（EC-SOD）, involved in the defense system against reactive oxygen species（ROS）. The two SOD...
关键词：Extracellular superoxide dismutase Protein fusion Heterologous expression PICHIAPASTORIS 
Expression and Characterization of HIV-1 Envelope Glycoprotein in Pichia Pastoris被引量：2
《Chemical Research in Chinese Universities》2008年第3期316-321,共6页ZHAO Li-hui YU Xiang-hui JIANG Chun-lai WU Yong-ge SHEN Jia-cong KONG Wei 
Jilin Basic Reesearch Program, China(No.20010710)
To obtain a sufficient amount of glycoprotein for further studying the structure and function of HIV-1 envelope glycoprotein, amplified and modified HIV-1 envelope glycoprotein gene which recombined subtypes（850 amin...
关键词：Pichia pastoris env gene GLYCOPROTEIN EPITOPE IMMUNOGENICITY 
Assembly and Immunogenicity of Human Papillomavirus Type 16 Major Capsid Protein(HPV16 L1) in Pichia pastoris
《Chemical Research in Chinese Universities》2007年第2期200-203,共4页LIU Da-wei ZHANG Yu YU Xiang-hui JIANG Chun-lai CHEN Yue WU Yong-ge JIN Ying-hua NIU Jun Qu Ning LIU Ming KONG Wei 
the National Natural Science Foundation of China(No20674029) ;the Science and Technology Department ofJilin Province(No20050402-4)
In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven by a strong AOX1 promoter. HPV16L1 gene was cloned into vector pPICZ,αB. HPV16 L1 protein expre...
关键词：Human papillomavirus Major capsid protein Recombinant Pichia pastoris Vires-like particles 
Expression and Characterization of Human Heart Type Fatty Acid Binding Protein in Pichia Pastoris
《Chemical Research in Chinese Universities》2006年第2期157-161,共5页HOU Wei TAN Yan XU Shu-fen YANG Xiao-hong ZHANG Shu-hua LIU Ling-li CHE Yuan-yuan LIU Li-hua 
SupportedbytheSocialDevelopmentProjectofScienceandTechnologyDepartmentofJilinProvince,China(No.20030450).
H-FABP is regarded as a tissue-specific protein existing only in myocardial cells. It is released from the cardiac tissue and gets into the plasma when a heart attack occurs; the myocardial infarction is a good case i...
关键词：Human heart type fatty acid binding protein Expression and purification PICHIA 
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