RENATURATION

作品数：15被引量：13H指数：2
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相关领域：生物学更多>>
相关作者：郭伟陈欣叶军钱世钧郭良栋更多>>
相关机构：中国科学院南京大学中国人民解放军郑州大学更多>>
相关期刊：《Cellular & Molecular Immunology》《Science China(Life Sciences)》《Science China Chemistry》《World Journal of Gastroenterology》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划“八五”国家科技攻关计划国家高技术研究发展计划更多>>
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Structure-activity correlationship and folding of recombinant Escherichia coli dihydro folate reductase (DHFR) enzyme through biochemical and biophysical approaches
《Journal of Biophysical Chemistry》2010年第2期105-112,共8页Jai Mittal Gayathri Ravitchandirane Tapan K. Chaudhuri Pratima Chaudhuri 
The design of any antagonist or inhibitor for any enzyme requires the knowledge of structure- function relationship of the protein and the optimum conformational states for maximum and minimum activities. Furthermore,...
关键词：Cellular FOLDING of E. coli DHFR STRUCTURE-FUNCTION Relationship Conformational Properties Equilibrium Unfolding Transitions Pathways for DENATURATION and RENATURATION 
Recombinant Expression of a Novel Human Transcriptional Repressor HMBOX1 and Preparation of Anti-HMBOX1 Monoclonal Antibody被引量：4
《Cellular & Molecular Immunology》2009年第4期261-268,共8页Jun Dai Longyan Wu Cai Zhang Xiaodong Zheng Zhigang Tian Jian Zhang 
Acknowledgements This work was supported by grants from National Natural Science Foundation of China （#30671901; #30628014）, National 863 Science Program by Ministry of Science and Technology of China （#2007AA021010, 2007AA021109）, National 973 Basic Science Project by Ministry of Science ＆ Technology of China （#2010CB911901）.
HMBOX1 was a novel transcription factor possibly involving in function of pancreas and cytotoxicity of NK cells. For function determination, recombinant human HMBOX1 protein was obtained and purified, and the monoclon...
关键词：HMBOX1 expression PURIFICATION RENATURATION monoclonal antibody 
Prokaryotic expression and purification of fibronectin leucine rich transmembrane protein 3 C-terminal domain proteins in rats
《Neural Regeneration Research》2009年第2期152-156,共5页Yan Cai Jing Yang He Huang Fang Li Ganqiu Wu Jing Yang Xuegang Luo 
Supported by:the National Natural Science Foundation of China,No.30600224;Supported by:the National Natural Science Foundation of China,No.30700438;China's Post-doctoral Science Fund, No.20060390886;Hunan Province Natural Science Foundation,No.06JJ30014; Hunan Province Scientific Program,No.2008FJ3138
BACKGROUND： Studies have suggested that fibronectin leucine-rich transmembrane protein 3 （FLRT3） is related to injury and regeneration of the nervous system. However, the expression and biological characteristics o...
关键词：FLRT3 fusion proteins isolation  purification and renaturation 
Effect of Denaturant Concentration on Hen-egg White Lysozyme Renaturation
《Chinese Journal of Chemistry》2006年第5期653-659,共7页边六交 杨晓燕 
Project supported by the Natural Science Foundation of Shaanxi Province （No. 2001K10-G3-3）.
Based on three-state renaturation process of denatured proteins, an equation describing the effect of denaturant concentration on renaturation yield of denatured proteins was presented. By this equation, two parameter...
关键词：egg white lysozyme UREA guanidine hydrochloride renaturation yield denaturant concentration 
Effect of ligand structure of stationary phase of high performance hydrophobic interaction chromatography on renaturation efficiency of GuHCl-denaturedα-chymotrypsin
《Science China Chemistry》2005年第z1期33-36,共4页SHEN Yehua WANG Haibo BAI Quan GENG Xindu 
supported by the National Natu-ral Science Foundation of China(Grant Nos.39880003&20175016).
The renaturation of the denaturedα-chymotrypsin(α-Chy)with 1.7 mol·L^(-1)guanidine hydrochloride(GuHCI)by three kinds of stationary phase of high performance hydrophobic interaction chromatography(STHIC)with a comp...
关键词：protein refolding RENATURATION Α-CHYMOTRYPSIN high performance hydrophobic interaction chromatography stationary phase 
Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma被引量：1
《World Journal of Gastroenterology》2004年第14期2029-2033,共5页Jin-LiangXing Xiang-MinYang Xi-YingYao FeiSong Zhi-NanChen 
Supported by the National High Technology Research and Development Program of China (863 Program),No.2001AA215101 and the National Natural Science Foundation of China,No.3020330
AIM: To express chimeric Fd (cFd) and chimeric light chain (cL) in E.coli respectively and refold them into chimeric Fab (cFab) antibody.METHODS: cFd and cL genes were respectively inserted into the prokaryotic expres...
关键词：原核表达 FAB抗体 肝细胞瘤 肿瘤 cL 
Increase Renaturation Yield of Reteplase Using the Recombinant Human Protein Disulfide Isomerase
《High Technology Letters》2004年第1期49-51,共3页赵友春  Wang Ge  Kong Yang  WANG Yanbing  Zhang Changkai  Chen Chao  Liang Bufeng  
Reteplase, the recombinant type of novel tissue plasminogen activator (t-PA) variant, is a promising thrombolytics in clinics. Expressed in the form of an inclusion body, reteplase consists of about 40 % of the total ...
关键词：人体组织 血浆酶原催化剂 硫蛋白异构酶 重建 复活产量 心脑血管疾病 溶血栓剂 
Studies on the Renaturation with Simultaneous Purification of Recombinant Human Proinsulin with Unit of Simultaneous Renaturation and Purification of Protein in Semi-preparative Scale
《Chinese Chemical Letters》2003年第8期824-827,共4页Quan BAI Yu KONG Xin Du GENG 
The renaturation and purification of recombinant human proinsulin (rh-proinsulin) expressed in E. coli with the unit of simultaneous renaturation and purification of protein (USRPP) in semi-preparative scale was studi...
关键词：Liquid chromatography hydrophobic interaction chromatography RENATURATION preparation recombinant human rh-proinsulin biotechnology. 
Comparison of the Contributions of Tetrahydrofurfuryl Alcohol and PEG to a-Chymotrypsin Renaturation with High Performance Hydrophobic Interaction Chromatography
《Chinese Chemical Letters》2003年第3期294-297,共4页Ye Hua SHEN Hai Bo WANG Quan BAI Xin Du GENG 
These projects No. 39880003 and 20175016 were supported by the National Natural Science Foundation of China.
The contributions of tetrahydrofurfuryl alcohol (THFA) and polyethylene glycol (PEG) to the renatured efficiency of a-chymotrypsin were investigated and compared with each other. The maximum increments of bioactivity...
关键词：High-perforamnce hydrophobic interaction chromatography tetrahydrofurfuryl alcohol (THFA) polyethylene glycol (PEG) protein renaturation a-chymotrypsin. 
In vitro renaturation of recombinant human pro-urokinase expressed in Escherichia coli被引量：1
《Chinese Medical Journal》2001年第2期74-78,110,共6页朱慧 刘伟 史蔚 薛宇鸣 蒯乐天 马忠 
theChineseNationalProgramofHigh TechnologyDevelopment !(No 863 10 2 0 8 0 2 0 1)
Objective　Recombinant human pro-urokinase forms insoluble inclusion body when overexpressed in Escherichia coli. It must be denatured and renatured in vitro so that it can acquire activity. This study aimed at increa...
关键词：recombinant human pro  urokinase  ·   denaturation   ·  renaturation 
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