TIRFM

作品数：8被引量：6H指数：2
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相关领域：生物学更多>>
相关作者：吴政星周围冯婉娟李臣鸿朱丹更多>>
相关机构：华中科技大学湖南大学中国科学院大学北京大学更多>>
相关期刊：《科学通报》《生物物理学报》《Chemical & Biomedical Imaging》《中国科学：生命科学》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划更多>>
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Driving Forces for Single DNA Stretching Assessed by In Situ TIRFM
《Chemical & Biomedical Imaging》2023年第2期140-146,共7页Jinli Han Ting Zhang Xiaochun Zhou 
support from the National Key R&D Program of China from the Ministry of Science and Technology(2020YFB1505700);Open Research Fund of Key Laboratory of the Ministry of Education for Advanced Catalysis Materials and Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces,Zhejiang Normal University(KLMEACM 202209);Suzhou Science and Technology Plan Project,Suzhou Carbon Peak Carbon Neutralization Technology Support Key Project(No.ST202214).
Herein,single DNA molecule stretching in a hydrodynamic flow was monitored by in situ total internal reflection fluorescence microscopy(TIRFM).Different driving forces for DNA stretching were assessed systematically.T...
关键词：DNA stretching TIRFM in situ imaging driving force hydrodynamic flow stretching 
Application of TIRFM in Biomolecule Research and Clinical Medicine
《Advances in Biological Chemistry》2021年第5期206-219,共14页Yexi Zhang 
With the increasing demand for imaging quality, many scientists constantly explore new imaging instruments to meet the requirements. The total internal reflection fluorescence micros...
关键词：TIRFM Single-Molecule Detection Dynamic Imaging Fluorescence Sensor 
The growth of B cell receptor microcluster is a universal response of B cells encountering antigens with different motion features被引量：1
《Protein & Cell》2012年第7期545-558,共14页Zhengpeng Wan Wanli Liu 
B lymphocyte cell senses and acquires foreign anti-gens through clonal distributed B cell receptors(BCRs)expressed on the surface of plasma membrane.The presentation formats of antigens are quite diverse.Based on thei...
关键词：BCR microcluster TIRFM live cell imaging fixed immobile antigen lateral mobile antigen free mobile antigen 
线虫中RAB-27与其效应物RBF-1调控致密核心囊泡的栓系和锚定过程
《中国科学：生命科学》2012年第5期365-373,共9页冯婉娟 梁滔 于俊伟 周围 章永登 吴政星 徐涛 
国家重点基础研究发展计划(批准号:30870564,90913022);国家自然科学基金(批准号:2010CB833701)资助项目
致密核心囊泡的转运、栓系、锚定和成熟过程的分子机制尚不清楚.本文以秀丽隐杆线虫(简称线虫)为模式生物来研究致密核心囊泡分泌过程中Rab蛋白及其效应物的功能.实验认为,RAB-27/AEX-6,而非RAB-3,是神经肽释放所必需的.通过全内反射荧...
关键词：分泌 致密核心囊泡 RAB-3 RAB-27 TIRFM 
Characterization of GLUT4-containing vesicles in 3T3-L1 adipocytes by total internal reflection fluorescence microscopy被引量：3
《Science China(Life Sciences)》2009年第7期665-671,共7页WANG Yan1,2,ZHANG JinZhong1,2,CHEN Yu1,2,JIANG Li1,2,JI Wei1,2 & XU Tao1 1 National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 
Supported by the National Natural Science Foundation of China (Grant Nos. 30470448 and 30130230);the National key Basic Research Program of China (Grant No. 2004CB720000);the Knowledge Innovative Program of The Chinese Academy of Sciences (Grant Nos. KSCX2-SW-224 and Y2004018);the Li Foundation and the Sinogerman Scientific Center
Insulin-responsive GLUT4(glucose transporter 4) translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle) poo...
关键词：GLUT4 IRAP-pHluorin GSV transferring TIRFM 
聚合物胶束细胞摄取的膜融合机制研究
《生物物理学报》2009年第S1期69-70,共2页肖玲 孙晓辉 陈华兵 朱艳红 徐辉碧 杨祥良 
973计划(2006CB705600);NSFC(30873121)资助
目的:聚合物胶束是一类具有临床应用前景的抗肿瘤药物纳米载药系统,其细胞摄取机理已引起广泛关注。本研究旨在阐明聚合物胶束的细胞摄取机理。
关键词：聚合物胶束 细胞摄取 膜融合 FRET TIRFM AFM 
Characterization of docking and fusion of synaptic-like microvesicles in PC12 cells using TIRFM
《Chinese Science Bulletin》2007年第22期3089-3096,共8页ZHOU Wei ZHU Dan LIANG Tao LI ChenHong WU ZhengXing 
Supported by the National Natural Science Foundation of China (Grant Nos. 30670502 and 30470646) ;the Major State Basic Research Program of China (Grant No. 2006CB503908)
Neurotransmitters are released by the fusion of synaptic vesicles with presynaptic membrane,which has been extensively studied. The analysis of single vesicle fusion kinetics reveals that there exist fusion modes of "...
关键词：PC12细胞 微泡 分析方法 神经传递素 
利用TIRFM技术检测PC12细胞中类突触小囊泡的锚定和融合特性被引量：2
《科学通报》2007年第19期2276-2282,共7页周围 朱丹 梁滔 李臣鸿 吴政星 
国家自然科学基金(批准号:30670502;30470646);国家重点基础研究发展计划(批准号:2006CB503908)资助项目
受到广泛关注的神经递质的释放是通过突触囊泡与突触前膜的融合完成的.通过对单个囊泡动力学的分析发现,在突触囊泡分泌过程中除了"完全融合"(full fusion)模式外,还存在着"部分融合即离开"(kiss and run)和"部分融合且停留"(kiss and s...
关键词：囊泡乙酰胆碱转运体 类突触小囊泡 VAMP2 胞吐 锚定 
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