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作品数：96被引量：166H指数：8
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A CRISPR/RfxCas13d-mediated strategy for efficient RNA knockdown in mouse embryonic development
《Science China(Life Sciences)》2024年第11期2297-2306,共10页Lin Zhang Shi-Meng Cao Hao Wu Meng Yan Jinsong Li Ling-Ling Chen 
supported by the Strategic Priority Research Program of the Chinese Academy of Science(XDB0570000);the CAS Project for Young Scientists in Basic Research(YSBR-009);the National Key Research and Development Program of China(2021YFA1100203,2020YFA0509000);the National Natural Science Foundation of China(31821004,32030029,32293230);Shanghai Municipal Science and Technology Major Project(23HC1401000,22YS1400900);support from the Xplorer Prize and New Cornerstone Science Foundation(NCI202232).
The growing variety of RNA classes,such as mRNAs,lncRNAs,and circRNAs,plays pivotal roles in both developmental processes and various pathophysiological conditions.Nonetheless,our comprehension of RNA functions in liv...
关键词：sperm-like stem cell semi-cloned technology CRISPR-RfxCas13d RNA knockdown embryonic development 
Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned,ASMT transgenic cloned dairy goats generated by the somatic cell nuclear transfer被引量：1
《Journal of Animal Science and Biotechnology》2023年第1期98-113,共16页Hao Wu Wendi Zhou Haijun Liu Xudai Cui Wenkui Ma Haixin Wu Guangdong Li Likai Wang Jinlong Zhang Xiaosheng Zhang Pengyun Ji Zhengxing Lian Guoshi Liu 
Key Research and Development Project of Hainan Province(ZDYF2021XDNY174);Science and Technology Major Project of Inner Mongolia(2021ZD0023–1);National Transgenic Key Project of the Ministry of Agriculture of China(2018ZX0800801B)。
Background:SCNT(somatic cell nuclear transfer)is of great significance to biological research and also to the livestock breeding.However,the survival rate of the SCNT cloned animals is relatively low compared to other...
关键词：Acetylserotonin-O-methyltransferase Dairy goat DNA methylation Gene editing Somatic cell nuclear transfer 
Analyses of the differential expression of cloned Caspase-3 and Caspae-9 genes,of Onchidium reevesii,exposed to extreme hot and cold conditions被引量：1
《Aquaculture and Fisheries》2021年第6期583-592,共10页Fengshen Zhao Heding Shen 
This work was supported by the Construction Project of the Double First-class Disciplines of Fisheries.
During low tide intertidal organisms face severe temperature fluctuations.This situation may be more pronounced in the context of climate change.To investigate the molecular response mechanism of Onchidium reevesii wh...
关键词：Onchidium reevesii CASPASE-3 CASPASE-9 Temperature Stress 
A framework for cloned vehicle detection被引量：1
《Frontiers of Computer Science》2020年第5期181-198,共18页Minxi Li Jiali Mao Xiaodong Qi Cheqing Jin 
Our research was supported by NSFC(Grant Nos.U1501252,U1711262,61702423 and U1811264).
Rampant cloned vehicle offenses have caused great damage to transportation management as well as public safety and even the world economy.It necessitates an efficient detection mechanism to identify the vehicles with ...
关键词：cloned vehicle detection object identification behavior pattern mining 
GbAt11 gene cloned from Gossypium barbadense mediates resistance to Verticillium wilt in Gossypium hirsutum
《Journal of Cotton Research》2020年第2期69-78,共10页QIU Tingting WANG Yanjun JIANG Juan ZHAO Jia WANG Yanqing QI Junsheng 
financially supported by the National Key Research and Development Program of China(2016YFD0101904)。
Background:Gossypium hirsutum is highly susceptible to Verticillium wilt,and once infected Verticillium wilt,its yield is greatly reduced.But G.barbadense is highly resistant to Verticillium wilt.It is possible that t...
关键词：Gossypium barbadense GbAt11 Verticillium wilt RESISTANCE 
Temporal regulation of prenatal embryonic development by paternal imprinted loci被引量：7
《Science China(Life Sciences)》2020年第1期1-17,共17页Qing Li Yuanyuan Li Qi Yin Shuo Huang Kai Wang Liangchai Zhuo Wei Li Boran Chang Jinsong Li 
partly supported by the Genome Tagging Project,Fountain-Valley Life Sciences Fund of University of Chinese Academy of Sciences Education Foundation and grants from the Chinese Academy of Sciences(XDB19010204,OYZDJ-SSW-SMC023 and Facility-based Open Research Program);the National Natural Science Foundation of China(31530048,81672117,31730062,31821004,and31601163);the Ministry of Science and Technology of China(2019YFA0109900);Shanghai Municipal Commission for Science and Technology(16JC1420500,17JC1420102,17JC1400900,and17411954900)
Paternal imprinted genes(H19 and Gtl2)are pivotal for prenatal embryonic development in mice.Nongrowing oocytes and sperm-or oocyte-originated haploid embryonic stem cells(ha ESCs)carrying both H19-DMR(differentially ...
关键词：imprinted loci semi-cloned technology temporal regulation H19-Igf2 Dlk1-Dio3 embryonic development 
Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA被引量：1
《World Journal of Pediatrics》2019年第5期499-505,共7页Duo Zhou Meng-Ying Zhu Yi-Long Wang Xiao-Qiang Hao Dong-Ming Zhou Rong-Xian Liu Chu-Di Zhang Chu-Fan Qu Zheng-Yan Zhao 
supported partly by the Natural Science Foundation for Young Scholars of Zhejiang Province(LQ19H100005).
Background Mumps is a common type of respiratory infectious disease caused by mumps virus(MuV),and can be effectively prevented by vaccination.In this study,a reverse genetic system of MuV that can facilitate the rati...
关键词：Mumps virus Reverse genetics PLASMID Safety IMMUNOGENICITY 
The Rise of Cloned Monkeys for Biomedical Studies
《Bulletin of the Chinese Academy of Sciences》2019年第1期14-15,共2页YAN Fusheng 
The biological differences between the widely used rodent models and humans have caused many fiascos in pharmaceutical development. Proving drugs effective in rodents does not guarantee they also work for humans.Unfor...
关键词：widely MODELS CASES 
Gene-edited Disease Monkeys Cloned at CAS
《Bulletin of the Chinese Academy of Sciences》2019年第1期57-57,共1页ION 
supported by grants from the Chinese Academy of Sciences;the Shanghai Municipal Government Bureau of Science and Technology;the Ministry of Science and Technology of China
The first cohort of five geneedited monkey clones made form fibroblasts of a monkey with disease phenotypes were born recently at the Institute of Neuroscience (10N) of the Chinese Academy of Sciences(CAS) in Shanghai.
关键词：COHORT SHANGHAI NEUROSCIENCE 
Comparison of birth weight and umbilical and placental characteristics of cloned and arti?cial insemination-derived piglets
《Frontiers of Agricultural Science and Engineering》2019年第1期54-60,共7页Zheng AO Chengfa ZHAO Yanmin GAN Xiao WU Junsong SHI Enqin ZHENG Dewu LIU Gengyuan CAI Zhenfang WU Zicong LI 
supported by two grants received from the Department of Science and Technology of Guangdong Province, China (2016B020233006 and 2016A020210074)
Somatic cell nuclear transfer(SCNT)-derived piglets have signi?cantly higher stillbirth rate and postnatal mortality rate than arti?cial insemination(AI)-generated piglets. The question whether the low survival rate o...
关键词：CLONED pig death PLACENTA SCNT UMBILICAL cord 
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