云南高校图书馆联盟文献共享服务平台- CRY1A

CRY1A: 作品数：92被引量：402H指数：9; 导出分析报告; 相关领域：农业科学更多>>; 相关作者：刘雨芳刘文海戈峰李菲张杰更多>>; 相关机构：中国农业科学院植物保护研究所东北农业大学湖南科技大学南京农业大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金转基因生物新品种培育专项国家科技重大专项国家重点基础研究发展计划更多>>

苏云金芽胞杆菌可视化快速表达载体的构建与特性分析: 《生物技术通报》2025年第1期95-102,共8页张静安胡孝龙曹蓓蓓廖敏束长龙张杰王奎操海群; 国家自然科学基金项目(32102295)。; 【目的】构建可以快速高效表达苏云金芽胞杆菌(Bacillus thuringiensis,Bt)杀虫基因的表达载体,提高Bt杀虫基因发掘及功能研究效率。【方法】以pUC18载体为基础,构建一个以cry1Ac基因启动子p1Ac指导、融合绿色荧光蛋白GFP的可视化快速...; 关键词：苏云金芽胞杆菌杀虫基因 cry1Ac启动子大肠杆菌表达载体

Mepiquat chloride increases the Cry1Ac protein content of Bt cotton under high temperature and drought stress by regulating carbon and amino acid metabolism: 《Journal of Integrative Agriculture》2024年第12期4032-4045,共14页Dian Jin Yuting Liu Zhenyu Liu Yuyang Dai Jianing Du Run He Tianfan Wu Yuan Chen Dehua Chen Xiang Zhang; supported by the National Natural Science Foundation of China(31901462);the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(22KJA210005);the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD);the Brand Professional Construction Program of Jiangsu Higher Education Institutions,China。; The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt ...; 关键词：amino acid metabolism Bt cotton carbon metabolism Cry1Ac protein mepiquat chloride

苏云金芽孢杆菌Cry1A.301-Vip3A融合杀虫蛋白原核表达及生物活性分析: 《南方农业学报》2024年第10期3046-3055,共10页杨小艳韩垚吴红雷开荣谢树章; 重庆市科研机构绩效激励引导专项(cstc2021jxjl80005);重庆市农业科学院绩效激励引导专项(cqaas2021jxjl24);重庆市农业科学院市级财政科研项目重大核心技术攻关项目(cqaas2023sjczhx016)。; 【目的】对苏云金芽孢杆菌(Bacillus thuringiensis,Bt)的杀虫蛋白基因(Cry1A.301和Vip3A)进行原核融合表达及生物活性分析,为玉米抗虫基因资源发掘和品种创制提供科学参考。【方法】利用连接肽构建不同杀虫机理的Cry1A.301和Vip3A融合...; 关键词：苏云金芽孢杆菌(Bt) 融合蛋白原核表达生物活性

转Cry1A.401基因抗虫玉米CM8302和CM8303对亚洲玉米螟的抗性研究: 《玉米科学》2024年第2期154-161,共8页王云鹤武奉慈谢树章翁建峰宋新元杨小艳李新海; 三亚崖州湾科技城管理局2020年度科技计划项目(SKJC-2020-02-005);重庆市农业科学院绩效激励引导专项项目(cqaas2021jxjl24)。; 采用农杆菌介导法获得转Cry1A.401基因抗虫玉米CM8302和CM8303,通过多代回交与自交获得BC_(4)F_(1)、BC_(4)F_(2)和BC_(4)F_(3)世代的转基因玉米材料。利用qRT-PCR和ELISA方法明确转基因抗虫玉米叶片、花丝和子粒均有Cry1A.401基因表达...; 关键词：转基因玉米亚洲玉米螟抗虫性遗传稳定性

A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops被引量：1: 《Food Science and Human Wellness》2024年第1期183-190,共8页Jinbin Wang Yu Wang Xiuwen Hu Yifan Chen Wei Jiang Xiaofeng Liu Juan Liu Lemei Zhu Haijuan Zeng Hua Liu; supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800);Shanghai Rising-Star Program(23QB1403500);the Shanghai Sailing Program(20YF1443000);Shanghai Science and Technology Commission,the Belt and Road Project(20310750500);Talent Project of SAAS(2023-2025);Runup Plan of SAAS(ZP22211);the SAAS Program for Excellent Research Team(2022(B-16))。; Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...; 关键词：Genetically modifi ed crops On-site detection Lateral fl ow test strips Dual recombinase polymerase amplification (RPA)

Selection,effective dominance,and completeness of Cry1A.105/Cry2Ab2 dual-protein resistance in Helicoverpa zea(Boddie)(Lepidoptera:Noctuidae): 《Journal of Integrative Agriculture》2023年第7期2151-2161,共11页Tiago SILVA Ying NIU Tyler TOWLES Sebe BROWN Graham PHEAD Wade WALKER Fangneng HUANG; This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript No.2022-234-37238;This project represents work supported by Bayer Crop Science(St.Louis,MO,USA);the Hatch funds from the USDA National Institute of Food and Agriculture,and the USDA Regional Research Project NC-246.; In the U.S.,Helicoverpa zea(Boddie)is a major pest targeted by both transgenic maize and cotton expressing Bacillus thuringiensis(Bt)proteins.Resistance of insect to Bt maize and cotton containing cry1A and cry2A gene...; 关键词：corn earworm Cry1A/Cry2A effective dominance incomplete resistance Bt maize resistance management

Relative fitness of susceptible and Cry1A.105/Cry2Ab2-single-/dual-protein-resistant Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) on non-Bt diet and a diet containing a low concentration of two proteins: 《Insect Science》2023年第2期398-410,共13页Shucong Lin Graham Head Paula Price Ying Niu Fangneng Huang; supported by Bayer Crop Science(St.Louis,MO,USA),USDA Regional Research Project NC-246;Hatch funds from the USDA National Institute of Food and Agriculture.; Helicoverpa zea(Boddie)is a destructive agricultural pest species that is targeted by both Bacillus thuringiensis(Bt)maize and cotton in the United States.Cry1A.105 and Cry2Ab2 are two Bt proteins expressed in a widel...; 关键词：resistance management Cry1A.105 Cry2Ab2 fitness costs Helicoverpa zea low dose effect

转基因抗虫耐除草剂玉米瑞丰125 Cry1Ab/Cry2Aj杀虫蛋白的时空表达分析被引量：3: 《生物技术通报》2023年第1期31-39,共9页李东阳肖冰王晨尧杨现明梁晋刚吴孔明; 转基因生物新品种培育重大专项(2019ZX08012-004);农业科研杰出人才培养计划(2021年)。; 玉米是我国最重要的粮食作物之一,虫害会造成严重的品质和产量损失,转Bacillus thuringiensis(Bt)基因抗虫玉米为玉米害虫的防治提供了新途径。转Bt基因抗虫玉米的抗虫性与外源杀虫蛋白表达量具有密切的关系,明确Bt杀虫蛋白在不同生育...; 关键词：转基因抗虫玉米瑞丰125 Cry1Ab/Cry2Aj杀虫蛋白时空表达

转cry1A.301基因玉米对主要鳞翅目害虫的抗性研究被引量：3: 《农业生物技术学报》2022年第7期1247-1256,共10页杨小艳王云鹤翁建峰吴红韩垚雷开荣谢树章; 重庆市农业科学院农业发展资金项目(NKY-2021AB001)。; 亚洲玉米螟(Ostrinia furnacalis)、黏虫(Mythimna separata)和棉铃虫(Helicoverpa armigera)是为害玉米(Zea mays)的主要鳞翅目害虫。cry1A.301基因是经优化的抗虫基因。为探讨转cry1A.301基因玉米对亚洲玉米螟、黏虫和棉铃虫的抗性,...; 关键词：cry1A.301基因抗虫玉米鳞翅目害虫抗虫性

Significant sensing performance of an all-silicon terahertz metasurface chip for Bacillus thuringiensis Cry1Ac protein被引量：7: 《Photonics Research》2022年第3期740-746,共7页Zijian Cui Yue Wang Yongqiang Shi Yongqiang Zhu Dachi Zhang Zhiqi Hong Xuping Feng; Natural Science Foundation of Shaanxi Province(2020JZ-48);Youth Innovation Team of Shaanxi Universities(21JP084);National Natural Science Foundation of China(31801257,61975163);Open Project of Key Laboratory of Engineering Dielectrics and Its Applications,Ministry of Education(KEY1805).; The promising prospect of a terahertz metasurface in sensing and detection applications has attracted increasing attention because of its ability to overcome the classical diffraction limit and the enhancement of fiel...; 关键词：stability process OVERCOME

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