MAMMALIAN_CELLS

作品数：89被引量：390H指数：7
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Improving cooperativity of transcription activators by oligomerization domains in mammalian cells
《Synthetic and Systems Biotechnology》2023年第1期114-120,共7页Xinmao Chen Ziming Liu Chunbo Lou Ying Guan Qi Ouyang Yanhui Xiang 
supported by Ministry of Science and Technology of China [No.2021YFA0910700,2021YFF1200500,2020YFA0907101];the Natural Science Foundation of China [No.12090050,12090054,32071412];the Chinese Academy of Sciences [No.QYZDB-SSW-SMC050];CAS Youth Interdisciplinary Team and the Shenzhen Science and Technology Innovation Committee [No.JCYJ20180507182241844,JCHZ20200005,DWKF20190009].
Cooperative activation is critical for the applications of synthetic biology in mammalian cells.In this study,we have developed cooperative transcription factor by fusing oligomerization domain in mammalian cells.Firs...
关键词：ACTIVATION NARROW COOPERATIVE 
Microscale methods to assemble mammalian cells into tissue-like structures被引量：1
《Science China(Life Sciences)》2012年第10期862-871,共10页GONG PeiYuan ZHENG WenFu XIAO Dan JIANG XingYu 
supported by Ministry of Science and Technology of China(Grant Nos.2009CB930001 and 2011CB933201);Chinese Academy ofSciences(Grant No.KJCX2-YW-M15);the National Natural ScienceFoundation of China(Grant Nos.20890020,90813032,21025520 and 51073045)
Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for ...
关键词：microscale cell assembly tissue-like structures tissue engineering 
An integrated microfluidic device for long-term culture of isolated single mammalian cells被引量：2
《Science China Chemistry》2012年第4期502-507,共6页ZHENG ChunHong CHEN Gui’E PANG YuHong HUANG YanYi 
supported by the National Natural Science Foundation of China (20733001, 20890020, 90913011, 20905004);the Ministry of Science and Technology of China (2011CB809106);the Ministry of Education of China;the Fok Ying Tung Education Foundation
We developed an integrated microfluidic chip for long-term culture of isolated single cells. This polydimethylsiloxane (PDMS) based device could accurately seed each single cell into different culture chambers, and is...
关键词：MICROFLUIDICS single cell culture conditioned medium 
Toxic effects of Karenia mikimotoi extracts on mammalian cells被引量：2
《Chinese Journal of Oceanology and Limnology》2011年第4期860-868,共9页陈洋 颜天 于仁成 周名江 
Supported by the National Basic Research Program of China (973 Program) (No. 2010CB428700);the National Natural Science Foundation of China (Nos. U0733006, 40821004);the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX2-YW-Q07-02)
Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However, the pot...
关键词：Karenia mikimotoi mammalian ceils super-oxidation 
High-level expression, purification and characterization of codon-optimized recombinant hemagglutinin 5 proteins in mammalian cells被引量：1
《Chinese Medical Journal》2010年第8期1073-1077,共5页YANG Jing-lin WANG Hong-liang WANG Shun-xin YANG Peng LIU Kang-tai JIANG Cheng-yu 
This work was supported by the grants from the National Natural Science Foundation of China （No. 30625013, No. 30623009 and No. 30721063） and the Ministry of Science and Technology of China （No. 2009CB522105 and No. 2005CB523000）.
Background Numerous Asian cases of avian influenza virus infection, especially the highly pathogenic strain H5N1, in humans have raised the concern that another influenza pandemic is close. However, there are no effec...
关键词：HEMAGGLUTININ avian influenza protein purification mammalian cell expression 
Construction and identification of a vector expressing TRAM siRNA in mammalian cells
《Journal of Medical Colleges of PLA(China)》2005年第3期135-140,共6页陈力勇 朱佩芳 杨策 蒋建新 王正国 
SupportedbyMajorStateBasicResearchDevelopmentPro-gramofChina(G1999054203)
Objective:To construct and identify a vector expressing TRAM siRNA in mammalian cells.Methods:It was constructed that the vector named R-pSUPER-EGFP used to transcribe functional TRAM siRNA. Two of pair 64 nt TRAM gen...
关键词：TRAM SIRNA pSUPER-EGFP VECTOR mammalian cells 
Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore protein in mammalian cells
《Progress in Natural Science:Materials International》2003年第2期114-118,T001,共6页ZHUXiaodong YAOFeng TIENPo 
Supported by the Major State Basic Research Development Program of China (G1999075602)
A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. ...
关键词：tetracycline inducible expression system enhanced green fluorescent protein hepatitis B virus transdominant nega- tive proteins. 
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