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MAMMALIAN_CELLS: 作品数：89被引量：390H指数：7; 导出分析报告; 相关作者：贾浩李智华戚正武郭雪江李志广更多>>; 相关机构：麻省理工学院清华信息科学与技术国家实验室中国医学科学院北京协和医学院北京大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划中国博士后科学基金更多>>

Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells: 《Chinese Chemical Letters》2024年第9期219-222,共4页Yan Liu Yang Wang Jiayi Zhu Xuxian Su Xudong Lin Liang Xu Xiwen Xing; supported by the National Key R&D Program of China(Nos.2022YFC2804101,2020YFA0211200);National Natural Science Foundation of China(Nos.22377056,22222706,21977122)。; The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms,which has...; 关键词：RNA triplex PH-RESPONSIVE CRISPR/Cas9 Gene manipulation Eukaryotic cells

Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells: 《Cell Research》2024年第1期31-46,共16页Jiawei Shao Shichao Li Xinyuan Qiu Jian Jiang Lihang Zhang Pengli Wang Yaqing Si Yuhang Wu Minghui He Qiqi Xiong Liuqi Zhao Yilin Li Yuxuan Fan Mirta Viviani Yu Fu Chaohua Wu Ting Gao Lingyun Zhu Martin Fussenegger Hui Wang Mingqi Xie; supported by the HRHI program 202209009 of Westlake Laboratory of Life Sciences and Biomedicine,Westlake Education Foundation,Tencent Foundation,the National Natural Science Foundation of China(32071429);the Ministry of Science and Technology of China(2020YFA0909200);supported by China Postdoctoral Science Foundation(2020M671805,2020T130107ZX to J.S.).Some drawings in this manuscript were kindly provided by Scidraw.io.; Here,we present a gene regulation strategy enabling programmable control over eukaryotic translational initiation.By excising the natural poly-adenylation(poly-A)signal of target genes and replacing it with a syntheti...; 关键词：TRANSLATIONAL RENDER alone

Corrigendum to“Improving cooperativity of transcription activators by oligomerization domains in mammalian cells”[Synth Syst Biotechnol 8(1)(2023)114-120]: 《Synthetic and Systems Biotechnology》2023年第4期771-771,共1页Xinmao Chen Ziming Liu Chunbo Lou Ying Guan Qi Ouyang Yanhui Xiang; The authors regret that following the publication of the original article,the authors noticed that Funds in Acknowledgements section was incorrect,in which a fund(No.2020YFA0906903)supporting most of experimental resu...; 关键词：SUPPORTING CORRECT sincerely

Improving cooperativity of transcription activators by oligomerization domains in mammalian cells: 《Synthetic and Systems Biotechnology》2023年第1期114-120,共7页Xinmao Chen Ziming Liu Chunbo Lou Ying Guan Qi Ouyang Yanhui Xiang; supported by Ministry of Science and Technology of China [No.2021YFA0910700,2021YFF1200500,2020YFA0907101];the Natural Science Foundation of China [No.12090050,12090054,32071412];the Chinese Academy of Sciences [No.QYZDB-SSW-SMC050];CAS Youth Interdisciplinary Team and the Shenzhen Science and Technology Innovation Committee [No.JCYJ20180507182241844,JCHZ20200005,DWKF20190009].; Cooperative activation is critical for the applications of synthetic biology in mammalian cells.In this study,we have developed cooperative transcription factor by fusing oligomerization domain in mammalian cells.Firs...; 关键词：ACTIVATION NARROW COOPERATIVE

Transcription-wide impact by RESCUE-induced off-target single-nucleotide variants in mammalian cells: 《Journal of Molecular Cell Biology》2023年第2期17-28,共12页Guo Li Xiaoning Zhu Yihan Wang Hongru Ma Yuzhe Wang Hanyu Wu Xiangyang Li Yiling Wang Jianen Gao Xuexin Chen Xingxu Huang Yuan Yao Xiaoxiang Hu; supported by grants from the Ministry of Agriculture of China(2016ZX08009003-006 and 2011ZX08006-001);the National Key Laboratory Open Fund Project(2020SKLAB6-24);the ZJU-Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University(02020200-K02013008);the National Natural Science Foundation of China(32071347).; RNA base editing is a promising tool in precise molecular therapy. Currently, there are two widely used RNA base editors, REPAIRand RESCUE. REPAIR only facilitates A-to-I conversions, while RESCUE performs both A-to-I...; 关键词：transcription-wide off-target SNV RESCUE RNA

Therapeutic cell engineering:designing programmable synthetic genetic circuits in mammalian cells被引量：3: 《Protein & Cell》2022年第7期476-489,共14页Maysam Mansouri Martin Fussenegger; Cell therapy approaches that employ engineered mam-malian cells for on-demand production of therapeutic agents in the patient's body are moving beyond proof-of-concept in translational medicine.The therapeutic cells c...; 关键词：synthetic biology cell-based therapy cell engineering therapeutic gene expression controllable genetic circuits

Nucleolus localization of SpyCas9 affects its stability and interferes with host protein translation in mammalian cells: 《Genes & Diseases》2022年第3期731-740,共10页Renke Tan Wenhao Du Yiyang Liu Xiaoji Cong Meirong Bai Chenxiao Jiang Zengxia Li Minjia Tan Dengke K.Ma Qiang Huang Wei Jiang Yongjun Dang; This work was supported by the National Key Research and Development Program of China(No.2018YFC0310900);the National Natural Science Foundation of China grants(No.31270830,21572038,21877016 to Y.D,No.31671386 to Q.H,81972621 to W.J);the Development Fund for Shanghai Talents,Fund of State Key Laboratory of Bioorganic and Natural Products Chemistry,Fund of State Key Laboratory of Drug Research,Chinese Academy of Science(No.SIMM1601KF-08);Fudan University Graduate Student Research Grant.; The CRISPR/Cas9 system,originally derived from the prokaryotic adaptive immune system,has been developed as efficient genome editing tools.It enables precise gene manipulation on chromosomal DNA through the specific b...; 关键词：CRISPR/Cas9 Gene editing Global transcription Nucleolus detention signal TRANSLATION

Regulation of mTORC1 by amino acids in mammalian cells: A general picture of recent advances被引量：7: 《Animal Nutrition》2021年第4期1009-1023,共15页Shizhe Zhang Xueyan Lin Qiuling Hou Zhiyong Hu Yun Wang Zhonghua Wang; supported by grants from the Modern Agroindustry Technology Research System of China(No.CARS-36);Taishan Scholar Project of Shandong Province。; The mechanistic target of rapamycin complex 1(mTORC1)integrates various types of signal inputs,such as energy,growth factors,and amino acids to regulate cell growth and proliferation mainly through the 2 direct downst...; 关键词：mTORC1 Amino acids LEUCINE MAMMAL Signaling pathway

Heightened protein-translation activities in mammalian cells and the disease/treatment implications被引量：3: 《National Science Review》2020年第12期1851-1855,共5页Chung-I Wu Haijun Wen; supported by the National Natural Science Foundation of China(31730046,91731000 and 81972691);the Guangdong Basic and Applied Basic Research Foundation(2020B1515020030);the National Key Research and Development Project(2020YFC0847000);the 985 Project(33000-18841204).; After cells divide,the new cells have to synthesize all the necessary cellular components in time for the next division.There is a lower bound of time required for cells to double their contents.For mammalian cells,th...; 关键词：dividing TREATMENT doubling

Heterologous expression and cell membrane localization of dinoflagellate opsins (rhodopsin proteins) in mammalian cells: 《Marine Life Science & Technology》2020年第3期302-308,共7页Minglei Ma Xinguo Shi Senjie Lin; The work was supported by the National Key Research and Development Program Grant(No.2017YFC1404302);Natural Science Foundation of China Grants NSFC(Nos.31661143029 and 41776116).; Rhodopsins are now found in all domains of life,and are classified into two large groups:type II,found in animals and type I found in microbes including Bacteria,Archaea,and Eukarya.While type II rhodopsin functions i...; 关键词：Dinoflagellate rhodopsins Proteorhodopsin-Subcellular localization HEK 293T cells

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