目的构建人凋亡抑制蛋白(human inhibitor of apoptosis protein2,hIAP-2)的shRNA,靶向抑制hIAP-2基因联合放疗,探讨其对CNE1鼻咽癌细胞株放射敏感性的影响。方法首先采用MTT法确定最佳放射剂量;再构建4条hIAP-2-shRNA沉默片段并用脂质...
目的:为了检测信号转导和转录活化蛋白2(signal transducer and activator of transcription factor 2,STAT2)基因的rs12422499和rs2066807两个单核苷酸多态性(single nucleotide polymorphism,SNP),建立一种简单、可靠的多聚酶链式反应...
supported by the National Natural Scientific Foundation of China (No. 30771122);National Undergraduate Innovation Training Project of Ministry of Education,China (No.AE11528)
本研究旨在探讨信号转导和转录活化蛋白4(signal transducer and activator of transcription 4,STAT4)受白介素-12(in-terleukin-12,IL-12)刺激后移位入细胞核的机制。对STATs家族成员进行同源性比对分析结果显示,STAT4的DNA结合域的39...