高速逆流色谱法分离茶黄素条件的优化  

Optimization of Separation Conditions of Theaflavins by High-speed Countercurrent Chromatography

作　　者：王坤波[1,2] 刘仲华[1,2] 黄建安[1,2] 刘芳[1,2] 龚雨顺[1,2] 潘宇[1,2] 黄浩[1,2] 

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]湖南农业大学湖南省天然产物工程技术研究中心,湖南长沙410128

出　　处：《食品科学》2009年第6期191-195,共5页Food Science

基　　金：科技部国际科技合作项目(2006DFA33220);"十五"国家科技攻关项目(2004BA542C)

摘　　要：优化高速逆流色谱分离4种茶黄素的方法。两相溶剂系统为正己烷-乙酸乙酯-甲醇-水-冰醋酸(1:5:1:5:0.25,V/V),固定相为体系的上相,下相为流动相,流速为2ml/min,仪器转速700r/min,进样量30mg。从茶黄素复合物中分离纯化得到茶黄素、茶黄素-3-没食子酸酯、茶黄素-3'-没食子酸酯和茶黄素-3,3'-双没食子酸酯,尤其使得两种酯溶性茶黄素(茶黄素-3-没食子酸酯、茶黄素-3'-没食子酸酯)达到较好的分离。In this study, high-speed countercurrent chromatography (HSCCC) was used to separate theaflavins. The optimized two-phase solvent system, flow rate, revolution speed and injection amount of HSCCC were hexane-ethyl acetate- methanol- water-acetic acid (1:5:1:5:0.25, V/V), 2 ml/min, 700 r/min and 30 mg, respectively. The upper phase was used as the stationary phase, while the lower phase was the mobile phase of HSCCC. Under these conditions, four theaflavins including theaflavin (TF1), theaflavin 3-gallate (T...

关 键 词：高速逆流色谱 分离 茶黄素 

分 类 号：R914[医药卫生—药物化学]