酶解制备牦牛乳酪蛋白降血压肽的初步研究  

Preliminary Studies on Preparation of Antihypertensive Peptides from Yak Milk Casein Using Enzymic Hydrolysate

作　　者：李倬[1] 李明生[1] 田伟[1] 张福梅 李云龙[1] 靳冬武 马忠仁[1,3] 冯玉萍 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学实验中心,甘肃兰州730030 [3]兰州民海生物工程有限公司,甘肃兰州730010

出　　处：《西北民族大学学报（自然科学版）》2013年第1期32-37,共6页Journal of Northwest Minzu University(Natural Science)

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2011-17);校地合作项目<牦牛乳的综合开发与利用>(XBMU-2011-BC-42);甘肃省科技支撑计划项目<水解乳蛋白的制备及产业化>(2010-147号)

摘　　要：目的:牦牛乳富含酪蛋白,其经酶解制备的降血压肽以安全性高、无副作用等优点已成为乳品开发和高血压防治药物研究的新热点.方法:本实验对从牦牛乳中提取的酪蛋白,依据复合蛋白酶、中性蛋白酶、碱性蛋白酶、木瓜蛋白酶及胰蛋白酶各自的最适pH及温度采用酶解方法制备降血压肽,并且以ACE抑制活性为指标初步筛选最佳水解酶.结果:五种酶ACE抑制肽抑制率分别为:复合蛋白酶为39.02%,中性蛋白酶为67.48%,碱性蛋白酶为56.10%,木瓜蛋白酶为71.54%,胰蛋白酶为8.94%.结论:说明,在最适pH及温度条件下,木瓜蛋白酶和中性蛋白酶为制备降血压肽的最佳酶选,这为后续进一步的制备降血压肽实验提供依据.Objective In order to explore a effective method for preparing antihypertensive peptides yak milk casein.Method Based on optimum pH and temperature of the complex protease,neutral protease and trypsin,and alkaline protease and papain enzyme respectly,the experiment took antihypertensive peptides preparation methods to prepare antihypertensive peptides and had the preliminary selection of optimum hydrolysis enzyme in accordance to ACE inhibitory activity.Results The inhibition rate of five ACE inhibition peptides enzyme(including composite protease,neutral protease,alkaline protease,and trypsin) were 39.02%,67.48%,56.1%,71.54% and 8.94% respectively.Conclusion The neutral protease and papain enzymes were the best selection to prepare antihypertensive peptides under the optimum pH and temperature conditions,which provides a experimental basis for subsequent further preparation of antihypertensive peptides.

关 键 词：牦牛乳 酪蛋白 ACE 降血压肽 

分 类 号：Q51[生物学—生物化学] TS252[轻工技术与工程—农产品加工及贮藏工程]