化学发光免疫分析技术及其在食品安全检测中的研究进展  

Research progress on chemiluminescence immunoassay technique and its application in food safety detection

作　　者：张燕[1] 杨金易[1] 曾道平[1] 易娜[1] 孙远明[1] 

机构地区：[1]华南农业大学食品学院广东省食品质量安全重点实验室农业部农产品贮藏保鲜质量安全风险评估实验室,广州510642

出　　处：《食品安全质量检测学报》2013年第5期1421-1427,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31201361);广东省科技计划项目(2010A032000001-4;2012A020100002);广州市科技计划项目-珠江新星项目(1217000380);星火计划(2012GA780001)~~

摘　　要：近年来,化学发光免疫分析因其具有特异性强、灵敏度高、线性范围宽、仪器简单、操作方便、无放射性污染等优点越来越受到人们的关注。本文简要介绍了化学发光免疫分析的类型及其原理,根据化学发光体系中标记物质的不同以及检测体系的差别,对化学发光免疫分析技术进行了系统的分类,并且对各种方法进行了详细的介绍,对其优点和不足做了透彻的分析。对该分析方法在食品安全检测中的运用,包括检测食品中微生物、毒素、农药残留、兽药残留等进行了综述,并展望其发展方向。Chemiluminescence immunoassay (CLIA) received more and more attention in recent years for its high sensitivity, wide linear range, simplicity, feasibility and non-isotopic contamination. The classification and principle of chemiluminescence immunoassay were introduced briefly in this article, and the CLIAs were classified systematically according to different marker substances of the chemiluminescence system and the difference of detection systems. A variety of methods were also introduced in detail. Moreover, its advantages and disadvantages were analyzed and the application of CLIA in food safety detection, including the detection of microorganism, toxins, pesticide residues, animal drug residues and the genetically modified food were dis-cussed. The future development of CLIA was also prospected.

关 键 词：化学发光免疫分析 食品安全 检测 应用 

分 类 号：R155.5[医药卫生—营养与食品卫生学]