红曲黄色素的分离、纯化及光稳定性探讨  

Investigation on the Separation Purification and Light Stability of Monascus Yellow Pigment

作　　者：代春华[1] 邓思珊 甘纯玑[1] 

机构地区：[1]福建农林大学生物技术中心,福州350002 [2]福建省医药科学研究所,福州350001

出　　处：《中国食品学报》2004年第3期63-67,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省科技厅资助项目(No.98-Z-100)

摘　　要：为深入研究红曲菌代谢产物生成过程及其用途,采用柱层析法对红曲中的醇溶性黄色素组分进行分离和纯化,并用高效液相色谱对其纯度及光稳定性进行表征。研究结果表明,利用柱层析法可以分离和纯化醇溶性红曲提取物中的黄色素,其最大吸收峰为385nm。红曲黄色素对光较敏感,光照后由于其分解使分子结构发生变化,导致褪色,因此对红曲黄色素应避光保存。In order to investigate the formative process and applicational exploitation of Monascus metabolized products the fraction of alcohol soluble yellow pigment was separated and purified by column chromatography and its purity and light stability were characterized by the HPLC. Experimental results showed that the column chromatography was suitable to separate and purify the yellow pigment in the alcohol-soluble Monascus extractive the maximum absorption peak of the pigment was at 385 nm. The Monascus yellow pigment was sensitive of light the some changes of its molecular structure was found after the light illumination due to decomposition reaction it resulted in the decoloration of the pigment. Thus the Monascus yellow pigment would be stored in light proof condition.

关 键 词：红曲 柱层析法 研究结果 分离和纯化 提取物 高效液相色谱 黄色素 光稳定性 并用 组分 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ925.7[轻工技术与工程—食品科学与工程]