变性豆粕康氏木霉固态发酵及酶水解的研究  

Study on Solidity Fermentation and Enzyme Hydrolysis of Defatted Soybean Meal

作　　者：石长波[1] 徐忠[1] 杨铭铎[1] 

机构地区：[1]哈尔滨商业大学,黑龙江哈尔滨150076

出　　处：《食品科学》2004年第11期210-212,共3页Food Science

摘　　要：为了充分利用变性脱脂豆粕资源,对脱脂豆粕固态发酵产酶及酶水解过程影响因素进行了研究,影响康氏木霉固态发酵的主要因素为温度、pH值、培养基液固质量比及培养时间,影响纤维素酶水解的条件主要为温度、pH值及时间,结果表明:较适宜的产酶条件是温度为30℃,pH值 4.5,培养基液固质量比2.5:1 时间为4d,产纤维素酶活力为7.98U/g,以所产纤维素酶进行酶水解,较适宜条件为:温度50℃、pH值5.0、时间3d、还原糖为5.32%。 研究结果为变性脱脂豆粕进一步开发利用利用提供了参考数据。For making use of defatted soybean meal,the influence factor during the cellulase produced by trichoderma koningiisolidity fermentation and cellulase enzyme hydrolysisof defatted soybean meal. The major affecting factors of solidityfermentation are temperature , pH , medium moisture content and time.The major affecting factors of enzymatic hydrolysis arepH value, temperature and time .It showed that the adequate condition for enzyme production is temperature (30℃), pH (4.5),medium moisture content 2.5:1, time (4d), and cellulase activity produced is 7.98U/g. And we also proceeded enzyme hydrolysisby cellulase produced, the optimum condition is temperature 50℃, pH 5.0, time3d, the yield of enzyme hydrolysis is 5.32%.

关 键 词：酶水解 变性脱脂豆粕 纤维素酶 固态发酵 pH值 参考数据 还原糖 康氏木霉 温度 培养基 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] S816[轻工技术与工程—食品科学与工程]