绿色木霉固态发酵纤维素酶生物量的测定及发酵培养基的初步优化  

Trichoderma viride Biomass Assay in the Solid Fermentation of Cellulase and the Preliminary Optimization of the Medium

作　　者：姜绪林[1] 孙瑶[1] 杨毅[1] 杜大庆[1] 张星元[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《食品与生物技术学报》2005年第3期101-105,共5页Journal of Food Science and Biotechnology

摘　　要：为了测定绿色木霉固态发酵纤维素酶的生物量,采用紫外分光检测固态发酵过程中麦角固醇的量.确定了其最佳提取工艺:在75℃恒温水浴中反应0.5h,用乙醚萃取,乙醇定容,紫外282nm检测.在此基础上对绿色木霉固态发酵的培养基进行了初步的优化,并得到了最佳的培养基组合:碳源为蔗糖,无机氮源为(NH4)2SO4,水料质量比为2.2,pH为5.5.用该培养基,CMC酶活和滤纸酶活分别比优化前提高了21.0%和44.1%.In order to obtain the data of the biomass in the solid cellulase fermentation, the method of biomass assay was firstly studied. The optimal extraction condition of ergosterol(an element in the mycelium wall) was determined as following: reacting at 75℃ for 0. 5 h, then the reaction liquid was extracted with ether, solved with 10 mL ethanol, and detected at 282 nm by UV spectrophotometer. On the basis of this biomass assay, the medium for the solid fermentation was optimized: sugar was used as the carbon source, and (NH4)2SO4 was used as the inorganic nitrogen source, the mass ratio of water to solid material was 2. 2, and the initial pH was 5. 5. The enzyme activities using CMC-Na and filter paper as the substrate increased by 21. 0% and 44.1%, respectively.

关 键 词：绿色木霉 固态发酵 纤维素酶 生物量 麦角固醇 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]