铁棍山药PPO的最适底物、热稳定性及褐变控制研究  

Studies on the Best Substrate,the Thermal Stability of Polyphenol Oxidase and the Browning Control in Dioscorea oppostia Thunb.cv.Tiegun

作　　者：刘莹[1] 赵喜亭 赵月丽[1] 鲁梦娇[1] 成淑君[1] 赵雪蕾[1] 李明军[1] 刘亚丽[1] Xi-ting Yue-li Meng-jiao Shu-jun Xue-lei Ming-jun Ya-li 

机构地区：[1]河南师范大学,生命科学学院,河南,新乡,453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,河南,新乡,453007

出　　处：《河南农业科学》2010年第12期95-98,共4页Journal of Henan Agricultural Sciences

基　　金：河南师范大学博士启动基金项目(051007);河南师范大学大学生创新性实验计划项目(2008421)

摘　　要：研究了铁棍山药多酚氧化酶(PPO)的最适底物、热稳定性及不同抑制剂对其活性的影响。结果表明:在试验范围内,铁棍山药PPO最适底物为邻苯二酚;在较低温度(20～40℃)下其PPO能保持较稳定的活性,较高温度(40～80℃)下其活性快速下降,80℃下保温10 min,PPO活性几乎为零;抑制剂对PPO活性的抑制效果依次为:L-抗坏血酸>L-半胱氨酸盐酸盐>植酸>柠檬酸。The best substrate,the thermal stability of polyphenol oxidase(PPO) from Dioscorea oppostia Thunb.cv.Tiegun and the effects of inhibitor on its activity were studied in this paper.The results showed that: The best substrate of PPO from Dioscorea oppostia Thunb.cv.Tiegun was catechol in the range of this experiment;the activity of PPO was more stable under the lower temperature(20-40℃),while,it's avtivity declined rapidly under the higher temperature(40-80℃)and was inhibited almost completely when incubated for 10min under 80℃;the order of inhibition effect from strong to weak was:L-ascorbic acid,L-cysteine hydrochloride,phytic acid,citric acid.

关 键 词：铁棍山药 PPO活性 底物 热稳定性 褐变 控制研究 Studies Control POLYPHENOL Oxidase 半胱氨酸盐酸盐 抑制剂 多酚氧化酶 抑制效果 试验范围 邻苯二酚 抗坏血酸 较低温度 柠檬酸 高温度 植酸 

分 类 号：S[农业科学]