离子色谱法测定食品中的甜蜜素和苯甲酸  

Determination of sodium cyclamate,benzoic acid in food by ion chromatography

作　　者：钟志雄[1] 梁春穗[1] 姚敬[1] 范山湖[1] 许秀敏[1] 

机构地区：[1]广东省疾病预防控制中心,广州510300

出　　处：《中国卫生检验杂志》2005年第9期1062-1064,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:采用离子交换-电导检测离子色谱法测定食品中的甜蜜素和苯甲酸.方法:选用IonPacAS11-HC分析柱,25.0 mmol/L NaOH+5% 甲醇淋洗液,外接水自动再生,样品经沉淀处理,过滤后进样分析.结果:方法的线性范围广、相关性好( r >0.999),甜蜜素和苯甲酸样品及标准测定的相对标准偏差(RSD)分别为 0.2%～4.8%、3.5%、0.1%～7.6%、0.6%,样品加标回收率分别为 90.4%～105.1%、92.0%～106.8%,检出限分别为 0.48、0.57 mg/kg.结论:与国标方法对比测定的结果表明,该方法的准确度高,适用性较好.Objective：To detect sodium cyclamate, benzoic acid in food by ion chromatography. Methods： InPacAS11 - HC was selected as the separation column,25 mmol/L Na2CO3 ＋ 5% Methanol was eluent, and the instrument auto-suppression with external water mode. After deposited and filtrated with 0. 45μm membrane, the sample were injected and analysized. Results：This method had a broad linear, a good relativity（ r 〉 0. 999） , the relative standard deviation （RSD） of sodium cyclamate and benzoic acid of standards and samples were 0. 2% - 4. 8%, 3.5%, 0. 1% - 7.6%, 0. 6%, respectively. The average rates of recoveries were between 90.4% and 105. 1% ,92.0% and 106. 8% ,and the method detection limit was 0.48,0. 57 mg/kg. Condusion： This method has better accuracy and adaptility compared with the results of GC and HPLC method.

关 键 词：离子色谱法 食品 甜蜜素 苯甲酸 

分 类 号：R155.5[医药卫生—营养与食品卫生学]