L-嗜热乳酸杆菌T-1的5L发酵罐的发酵条件研究  

Study on L-Lactobacillus thermophilus T-1 fermentation in 5L fermenter

作　　者：高年发[1] 苏敬红[1] 张健[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300022

出　　处：《中国酿造》2006年第3期11-14,共4页China Brewing

摘　　要：对L-嗜热乳酸杆菌T-1在5L发酵罐上进行了分批发酵和补料分批发酵的条件研究。实验结果表明:分批发酵最适条件为以Na2CO3作中和剂,温度50℃;pH6.0,溶氧范围为前0h￣20h通风1L/min,搅拌转速100r/min,20h后停止通风和搅拌,产乳酸95g/L,产酸速率1.32g/(L.h),转化率92.7%。补料分批发酵实验表明,2次补料乳酸产量明显高于1次补料,产酸117.5g/L,转化率90.4%。The conditions of batch and fed-batch fermentation by L-Lactobacillus thermophilus T-1 in a 5 L fermenter were studied. The production yield of lactic acid reached 95 g/L with a rate of 1.32 g/（L h）, and a conversion rate of 92.7% with conditions ofpH 6.0, temperature 50℃ and the dissolved oxygen controlled by aeration 1 L/min and stirring 100 r/min for 20 h. Furthermore, this article also shows that two times fed-batch was better than one time fed-batch, the production amount of lactic acid was up to 117.5 g/L and the conversion rate was 90.4%.

关 键 词：L-乳酸 发酵 嗜热乳酸杆菌 

分 类 号：TQ921[轻工技术与工程—发酵工程]